Counter-proliferative effects of nucleosomal antigens in cultures from lupus patients
Autor: | Myer R. Salaman, M H Seifert, D Pc Mawer, David A. Isenberg, M B Hogarth |
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Rok vydání: | 2001 |
Předmět: |
Adult
Male T cell DNA Single-Stranded Stimulation Lymphocyte proliferation 030204 cardiovascular system & hematology Biology Peripheral blood mononuclear cell Histones 03 medical and health sciences 0302 clinical medicine Rheumatology Antigen medicine Humans Lupus Erythematosus Systemic Cells Cultured 030203 arthritis & rheumatology Systemic lupus erythematosus Tuberculin Test Pokeweed mitogen DNA Middle Aged medicine.disease Molecular biology Nucleosomes medicine.anatomical_structure Histone Pokeweed Mitogens Immunology Leukocytes Mononuclear biology.protein Female Cell Division |
Zdroj: | Lupus. 10:332-339 |
ISSN: | 1477-0962 0961-2033 |
Popis: | Blood mononuclear cells from 20 lupus patients were cultured in the presence of nucleosomal antigens to determine whether they induce lymphocyte proliferation. The predominant effect seen, however, was one of inhibition of the background proliferation. Such inhibition was rare with cells from female or male controls. Nucleohistone (NH), crude histone and enriched preparations of histones H2A/H4, H2B and H3 showed this effect in approximately one-third of patients, but H1 and single-stranded (ss) DNA had no such activity. Double-stranded (ds) DNA may show this inhibitory action, but further tests are required. ssDNA was the only antigen that showed evidence (two patients) of disease-related stimulation of proliferation. Histones and NH induced proliferation in many subjects but the strongest responders were controls. Patients responded poorly to tuberculin PPD but gave an exceptionally strong proliferative response to pokeweed mitogen. It is suggested that the inhibition of background proliferation in patients is a consequence of the interaction of nucleosomal antigens with sensitised T cells. If T cell sensitisation to histones is an important factor in the development of lupus, the disease may be preventable in those at risk by inducing tolerance to the appropriate peptides. |
Databáze: | OpenAIRE |
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