Transposase-dependent formation of circular IS256 derivatives in Staphylococcus epidermidis and Staphylococcus aureus
| Autor: | Jörg Hacker, Wilma Ziebuhr, Isabel Loessner, Dorothea Dittrich, Katja Dietrich |
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| Rok vydání: | 2002 |
| Předmět: |
Genetics
Staphylococcus aureus Bacteriophages Transposons and Plasmids Transposases Biology Extrachromosomal circular DNA medicine.disease_cause biology.organism_classification Microbiology law.invention Open reading frame Plasmid Staphylococcus epidermidis law medicine Recombinant DNA DNA Transposable Elements Humans Insertion sequence Molecular Biology Escherichia coli Transposase |
| Zdroj: | Journal of bacteriology. 184(17) |
| ISSN: | 0021-9193 |
| Popis: | IS 256 is a highly active insertion sequence (IS) element of multiresistant staphylococci and enterococci. Here we show that, in a Staphylococcus epidermidis clinical isolate, as well as in recombinant Staphylococcus aureus and Escherichia coli carrying a single IS 256 insertion on a plasmid, IS 256 excises as an extrachromosomal circular DNA molecule. First, circles were identified that contained a complete copy of IS 256 . In this case, the sequence connecting the left and right ends of IS 256 was derived from flanking DNA sequences of the parental genetic locus. Second, circle junctions were detected in which one end of IS 256 was truncated. Nucleotide sequencing of circle junctions revealed that (i) either end of IS 256 can attack the opposite terminus and (ii) the circle junctions vary significantly in size. Upon deletion of the IS 256 open reading frame at the 3′ end and site-directed mutageneses of the putative DDE motif, circular IS 256 molecules were no longer detectable, which implicates the IS 256 -encoded transposase protein with the circularization of the element. |
| Databáze: | OpenAIRE |
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