Activity-Dependent Presynaptic Effect of Serotonin 1B Receptors on the Somatosensory Thalamocortical Transmission in Neonatal Mice

Autor: Olivier Cases, Patricia Gaspar, Nicole Ropert, Cécile Lebrand, Jean-Marc Goaillard, Alban Laurent
Rok vydání: 2002
Předmět:
Agonist
Aging
Serotonin
Patch-Clamp Techniques
Pyridines
medicine.drug_class
Aging/metabolism Animals Animals
Newborn Electric Stimulation Excitatory Postsynaptic Potentials/drug effects/physiology Mice Mice
Inbred Strains Mice
Knockout Neural Inhibition/drug effects Neurons/drug effects/metabolism Patch-Clamp Techniques Presynaptic Terminals/drug effects/*metabolism Pyridines/pharmacology Pyrroles/pharmacology RNA
Messenger/metabolism Receptor
Serotonin
5-HT1B Receptor
Serotonin
5-HT1D Receptors
Serotonin/deficiency/genetics/*metabolism Serotonin/pharmacology Serotonin Agonists/pharmacology Somatosensory Cortex/drug effects/*physiology Synaptic Transmission/drug effects/*physiology Thalamus/drug effects/growth & development/*physiology Ventral Thalamic Nuclei/growth & development/metabolism
Presynaptic Terminals
Mice
Inbred Strains
Stimulation
In Vitro Techniques
Biology
Neurotransmission
Somatosensory system
Synaptic Transmission
Mice
Thalamus
medicine
Animals
Pyrroles
RNA
Messenger
ARTICLE
Receptor
Mice
Knockout
Neurons
Ventral Thalamic Nuclei
General Neuroscience
Glutamate receptor
Excitatory Postsynaptic Potentials
Neural Inhibition
Somatosensory Cortex
Electric Stimulation
Serotonin Receptor Agonists
Animals
Newborn
Receptor
Serotonin
5-HT1D
Receptors
Serotonin
Receptor
Serotonin
5-HT1B
NMDA receptor
Neuroscience
Zdroj: Scopus-Elsevier
Europe PubMed Central
ResearcherID
Journal of Neuroscience, vol. 22, no. 3, pp. 886-900
ISSN: 1529-2401
0270-6474
Popis: The disruptive effect of excessive serotonin (5-HT) levels on the development of cortical sensory maps is mediated by 5-HT1B receptors, as shown in barrelless monoamine oxidase A knock-out mice, in which the additional inactivation of 5-HT1B receptors restores the barrels. However, it is unclear whether 5-HT1B receptors mediate their effect on barrel formation by a trophic action or an activity-dependent effect. To test for a possible effect of 5-HT1B receptors on activity, we studied the influence of 5-HT on the thalamocortical (TC) synaptic transmission in layer IV cortical neurons. In TC slices of postnatal day 5 (P5)–P9 neonate mice, we show that 5-HT reduces monosynaptic TC EPSCs evoked by low-frequency internal capsule stimulation and relieves the short-term depression of the EPSC evoked by high-frequency stimulation. We provide evidence that 5-HT decreases the presynaptic release of glutamate: 5-HT reduces similarly the AMPA–kainate and NMDA components and the paired pulse depression of TC EPSCs. We show also that 5-HT1B receptors mediate exclusively the effect of 5-HT: first, the effect of 5-HT on the TC EPSC is correlated with the transient expression of 5-HT1B receptor mRNAs in the ventrobasal thalamic nucleus during postnatal development; second, it is mimicked by a 5-HT1B agonist; third, 5-HT has no effect in 5-HT1B receptor knock-out mice. Our results show that in the developing barrel field of the neonatal mice, 5-HT1B receptors mediate an activity-dependent regulation of the TC EPSC that could favor the propagation of high-frequency TC activity.
Databáze: OpenAIRE
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