Distribution of the Mosaic Structured murM Genes among Natural Populations of Streptococcus pneumoniae
| Autor: | Elena Severina, Sergio R. Filipe, Alexander Tomasz |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
DNA
Bacterial Penicillin Resistance Molecular Sequence Data Cell Surfaces Peptidoglycan Biology medicine.disease_cause Microbiology Pneumococcal Infections Bacterial Proteins Sequence Homology Nucleic Acid Streptococcus pneumoniae medicine Pulsed-field gel electrophoresis Humans Amino Acid Sequence Peptide Synthases Molecular Biology Peptide sequence Gene Alleles Genetics Base Sequence Strain (chemistry) Hybridization probe Nucleic acid sequence Genes Bacterial Nucleic acid |
| Zdroj: | Journal of Bacteriology. 182:6798-6805 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | The presence and sequence variation of the murM gene were studied in a large collection (814 strains) of genetically diverse Streptococcus pneumoniae isolates, which included 27 different serogroups and both penicillin-resistant (423 isolates, 67 pulsed-field gel electrophoretic [PFGE] types) and intermediately penicillin-resistant (165 isolates, 66 PFGE types) and penicillin-susceptible (226 isolates, 135 PFGE types) strains. Diversity of the murM sequences was tested by hybridization with mainly two kinds of probes: one derived from the amplification of the nucleotide sequence between nucleotides 201 and 624 in the penicillin-susceptible laboratory strain R36A ( murMA probe) and a second probe that amplified the comparable, highly divergent sequence in the penicillin-resistant strain Pen6 ( murMB probe). The great majority of the strains (761 of 814), including both penicillin-susceptible and penicillin-resistant isolates, reacted exclusively with the murMA probe. A smaller group of penicillin-resistant strains (48 of 814 isolates) reacted only with the murMB DNA probe, and an additional 5 isolates reacted with both probes. High-pressure liquid chromatography analysis of the peptidoglycan of strains hybridizing with murMB showed that they invariably contained an increased proportion of branched peptides. Complete sequencing of murM from a group of penicillin-resistant isolates allowed the identification of a number of different murMB alleles that differed in the length and exact position of the divergent (Pen6 type) sequences within the particular murM . The close similarity of these divergent sequences in the various murM alleles suggests a possible common heterologous origin. |
| Databáze: | OpenAIRE |
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