HIV-1 cores retain their integrity until minutes before uncoating in the nucleus
| Autor: | Wei-Shau Hu, Ryan C. Burdick, Vinay K. Pathak, Kunio Nagashima, Chenglei Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
viruses
Human immunodeficiency virus (HIV) Cleavage and polyadenylation specificity factor medicine.disease_cause Microbiology gag Gene Products Human Immunodeficiency Virus Cell Line Green fluorescent protein capsid medicine Humans Nuclear pore Nucleocapsid core integrity Multidisciplinary Chemistry Biological Sciences Virus Internalization biochemical phenomena metabolism and nutrition nuclear import Nuclear environment Cell biology medicine.anatomical_structure Capsid HIV-1 uncoating Nuclear transport Nucleus |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.2019467118 |
| Popis: | Significance Here, we used a fluorescent protein that is free in solution and is trapped in nuclear HIV-1 capsids to demonstrate that the capsids retain integrity and prevent mixing of macromolecules within the viral core and the cellular environment until just before integration. We also found that capsid integrity is maintained until just minutes before disassembly in the nucleus, revealing that uncoating proceeds rapidly after integrity loss. These valuable insights into the early stage of HIV-1 replication indicate that intact HIV-1 capsids are imported through nuclear pores, that reverse transcription is mostly completed within intact capsids, and that preintegration complex-host interactions facilitating integration and target site selection must occur within a short time frame between capsid disassembly and integration. We recently reported that HIV-1 cores that retained >94% of their capsid (CA) protein entered the nucleus and disassembled (uncoated) near their integration site |
| Databáze: | OpenAIRE |
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