Undiscriminating codon reading with adenosine in the wobble position
| Autor: | Florentyna Lustig, Kenneth C. Kuo, Per Elias, Miroslawa Z. Barciszewska, Thomas Borén, Tore Samuelsson, Charles W. Gehrke, Catharina Claesson |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adenosine
Molecular Sequence Data Wobble base pair Biology Cell Line Capsid Structural Biology Escherichia coli Nucleotide Cloning Molecular Site-directed mutagenesis Codon Molecular Biology Gene chemistry.chemical_classification Genetics Chromatography Base Sequence Mycoplasma mycoides RNA-Binding Proteins Translation (biology) RNA Transfer Gly Genetic code chemistry Biochemistry Protein Biosynthesis Glycine Transfer RNA Mutagenesis Site-Directed Capsid Proteins |
| Zdroj: | Journal of molecular biology. 230(3) |
| ISSN: | 0022-2836 |
| Popis: | To investigate the reading properties of adenosine in the wobble position we have used site-directed mutagenesis of the Escherichia coli glycine tRNA 1 (CCC) gene to substitute the nucleotide A in the wobble position of the corresponding tRNA. The effect of this change on the ability of the tRNA to discriminate between the nucleotides in the third position of the glycine codons has been investigated. We have compared the ability of the mutant glycine tRNA 1 (UCC) and glycine tRNA 1 (ACC) as well as the mycoplasma glycine tRNA(UCC) to read the glycine codons. The results showed that glycine tRNA 1 (ACC) unlike glycine tRNA 1 (UCC) did not fully discriminate between the glycine codons. These experiments were carried out using a new in vitro protein synthesizing system that allows us to monitor the reading of all four glycine codons. In the present paper we give a detailed description of this new in vitro system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|