Rational design and characterisation of an amphipathic cell penetrating peptide for non-viral gene delivery
| Autor: | Nicholas Dunne, Vicky L. Kett, Emma M. McErlean, John W. McBride, Helen O. McCarthy, Grace Cole, Tracy Robson, Cian M. McCrudden |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Gene Transfer Techniques Rational design Pharmaceutical Science Peptide Cell-Penetrating Peptides Genetic Therapy 02 engineering and technology Transfection 021001 nanoscience & nanotechnology 030226 pharmacology & pharmacy RALA Amino acid 03 medical and health sciences 0302 clinical medicine chemistry Biochemistry Amphiphile Nucleic acid Cell-penetrating peptide Nanoparticles 0210 nano-technology Peptide sequence |
| Zdroj: | McErlean, E M, McCrudden, C M, McBride, J W, Cole, G, Kett, V L, Robson, T, Dunne, N J & McCarthy, H O 2021, ' Rational design and characterisation of an amphipathic cell penetrating peptide for non-viral gene delivery ', International Journal of Pharmaceutics, vol. 596, 120223 . https://doi.org/10.1016/j.ijpharm.2021.120223 |
| Popis: | RALA is a cationic amphipathic peptide which has shown great promise as an efficient, multifunctional delivery system for the delivery of nucleic acids. Rational peptide design was utilised in this study to understand the essential amino acids required for delivery and if any improvements to the RALA peptide could be made. Six amphipathic peptides were synthesised with strategic sequences and amino acid substitutions to reduce peptide sequence, while maintaining the functional characteristics of RALA including amphipathicity, alpha-helicity and pH responsiveness for endosomal escape. Data demonstrated that all six peptides complexed pEGFP-N1 to produce cationic nanoparticles |
| Databáze: | OpenAIRE |
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