Integrated identification, qualification and quantification strategy for pharmacokinetic profile study of Guizhi Fuling capsule in healthy volunteers
| Autor: | Guangji Wang, Yu Wang, Jiye Aa, Ke-Rong Zhang, Bingchen Ouyang, Jianguo Sun, Zhen-Zhong Wang, Xiaoliang Jin, Wei Xiao, Shiyin Gu, Ying Peng, Yunxi Zhong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Amygdalin Capsules Pharmacology 030226 pharmacology & pharmacy 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pharmacokinetics Glucosides In vivo Tandem Mass Spectrometry Gallic Acid Medicine Humans Gallic acid Chromatography High Pressure Liquid Guizhi-fuling Multidisciplinary Molecular Structure business.industry 010401 analytical chemistry Capsule Acetophenones Paeoniflorin Healthy Volunteers 0104 chemical sciences chemistry Cinnamates Monoterpenes Female Paeonol business Drugs Chinese Herbal |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Guizhi Fuling capsule (GZFL), a traditional Chinese medicine formulation, is widely used in China to relieve pain from dysmenorrhea and is now in a Phase II clinical trial in the USA. Due to the low exposure of the five main medicative ingredients (amygdalin, cinnamic acid, gallic acid, paeoniflorin and paeonol) of GZFL in human, a strategy was built to qualitatively and quantitatively identify the possible metabolites of GZFL and to describe the pharmacokinetic profiles of GZFL in human. In this strategy, LC-Q-TOF/MS was used to identify and structurally elucidate the possible metabolites of GZFL in vivo; and a time-based metabolite-confirming step (TBMCs) was used to confirm uncertain metabolites. The simultaneously quantitation results by LC-MS/MS showed low exposure of the five medicative ingredients. According to the strategy we built, a total of 36 metabolites were found and structurally elucidated. The simultaneously semi-quantitative analysis by LC-MS/MS showed that obvious time-concentration curves could be established for 12 of the metabolites and most of them showed a relatively higher exposure. This study provides a better understanding of the metabolic processes of GZFL in human. |
| Databáze: | OpenAIRE |
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