Nifedipine Prevents Sodium Caprate-Induced Barrier Dysfunction in Human Epidermal Keratinocyte Cultures
| Autor: | Ikuya Kimura, Takuya Watanabe, Masato Hamabashiri, Atsushi Yamauchi, Yoshihiro Uchino, Takashi Tsuchiya, Junichi Matsumoto, Yasufumi Kataoka |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Keratinocytes
medicine.medical_specialty Cell Membrane Permeability Nifedipine Pharmaceutical Science Pharmacology Tight Junctions Internal medicine Claudin-1 Electric Impedance medicine Humans Cells Cultured Transepidermal water loss Epidermal barrier integumentary system Tight junction Epidermis (botany) Chemistry Water General Medicine Endocrinology Sodium caprate Water metabolism Dermatologic Agents Epidermis Decanoic Acids medicine.drug Epidermal keratinocyte |
| Zdroj: | Biological & Pharmaceutical Bulletin. 38:926-929 |
| ISSN: | 1347-5215 0918-6158 |
| Popis: | Tight junctions (TJs) of the epidermis play an important role in maintaining the epidermal barrier. TJ breakdown is associated with skin problems, such as wrinkles and transepidermal water loss (TEWL). Clinical studies have reported that topical nifedipine is effective in reducing the depth of wrinkles and improving TEWL. However, it remains unknown whether nifedipine influences the TJ function in the epidermis. In the present study, we investigated the effect of nifedipine on epidermal barrier dysfunction in normal human epidermal keratinocytes (NHEKs) treated with sodium caprate (C10), a TJ inhibitor. Nifedipine reversed the C10-decreased transepithelial electrical resistance values as a measure of disruption of the epidermal barrier. Immunocytochemical observations revealed that nifedipine improved the C10-induced irregular arrangement of claudin-1, a key protein in TJs. Taken together, these findings suggest that nifedipine prevents epidermal barrier dysfunction, at least in part, by reconstituting the irregular claudin-1 localization at TJs in C10-treated NHEKs. |
| Databáze: | OpenAIRE |
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