A 10% Tomato Diet Selectively Reduces Radiation-Induced Damage in TRAMP Mice
| Autor: | John W. Erdman, Matthew A. Wallig, William D. O'Brien, Timothy M. Fan, Rita J. Miller, Joe L. Rowles, Kimberly A. Selting |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
H&E stain Medicine (miscellaneous) Mice Transgenic Andrology Mice Prostate cancer chemistry.chemical_compound Lycopene Solanum lycopersicum Prostate Animals Humans Medicine Nutrition and Dietetics business.industry Prostatic Neoplasms medicine.disease Diet medicine.anatomical_structure chemistry Apoptosis Duodenum Adenocarcinoma business Tramp |
| Zdroj: | The Journal of Nutrition. 151:3421-3430 |
| ISSN: | 0022-3166 |
| DOI: | 10.1093/jn/nxab257 |
| Popis: | BACKGROUND Tomatoes contain carotenoids that have the potential to alter the effects of external beam radiation therapy (EBRT). OBJECTIVES We hypothesized that dietary lyophilized tomato paste (TP) would reduce apoptosis within carotenoid-containing nonneoplastic tissues in EBRT-treated TRansgenic Adenocarcinoma of the Mouse Prostate (TRAMP) mice. METHODS Male TRAMP mice (n = 73) were provided an AIN-93G diet or a modified AIN-93G diet containing 10% TP (wt:wt) at 4 wk of age. Prostate tumor growth was monitored by ultrasound. The caudal half of the mouse was irradiated with 7.5 Gy (Rad) or 0 Gy (sham) at 24 wk of age or after the tumor volume exceeded 1000 mm3 with a Cobalt-60 source. Mice were euthanized 24 h postradiation. Carotenoids and α-tocopherol were measured by HPLC and compared by a t test. Tissues were assessed for radiation-induced changes (hematoxylin and eosin) and apoptosis [cleaved caspase-3 (CC3)] and compared by Kruskal-Wallis test or Freedman-Lane's permutation test. RESULTS Serum concentrations of lycopene (52% lower), phytoene (26% lower), and α-tocopherol (22% lower) were decreased in TP-fed irradiated mice (TP-Rad) compared with TP-fed sham mice (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|