Optimisation of pressurised water extraction of polysaccharides from blackcurrant and its antioxidant activity
| Autor: | Yu Yang, Yaqin Xu, Xingguo Li, Ling Zhang, Zeyuan Yu, Fei Cai, Gaijie Liu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Arabinose
Chromatography Gas Antioxidant Spectrophotometry Infrared Rhamnose DPPH medicine.medical_treatment 02 engineering and technology Xylose Polysaccharide 01 natural sciences Analytical Chemistry chemistry.chemical_compound Ribes Polysaccharides Pressure medicine Monosaccharide Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography Plant Extracts 010405 organic chemistry Monosaccharides Temperature Water Water extraction General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Molecular Weight chemistry 0210 nano-technology Oxidation-Reduction Food Science |
| Zdroj: | Food Chemistry. 194:650-658 |
| ISSN: | 0308-8146 |
| Popis: | Pressurised water extraction (PWE) of polysaccharides from blackcurrant fruits was investigated using a response surface methodology (RSM). The optimal conditions for PWE were: time 51min, pressure 1.6MPa, and temperature 52°C. Under these conditions, the experimental yield of Ribes nigrum L. polysaccharides (RNLP) was 11.68±0.12%, which closely agreed with the predicted value (11.77%). After preliminary purification with D4006 macroporous resin, RNLP I was obtained and its chemical characterisation was undertaken by GC, HPLC, and IR spectroscopy. RNLP I was composed of rhamnose, arabinose, xylose, mannose, galactose, and glucose with a molar ratio of 2.89:14.82:1.02:1.00:2.53:6.39 and its molecular weight was 1.49×10(4)kDa. The antioxidant activity of RNLP I was evaluated by free radical scavenging assays and a reducing power assay in vitro. RNLP I showed strong DPPH and superoxide radical scavenging activities and reducing power. |
| Databáze: | OpenAIRE |
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