Identification and characterization of a non-satellite cell muscle resident progenitor during postnatal development
| Autor: | Edgar R. Gomes, Kathryn J. Mitchell, David Sassoon, Alice Pannérec, Ara Parlakian, Vanessa Besson, Bruno Cadot, Giovanna Marazzi |
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| Rok vydání: | 2010 |
| Předmět: |
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Untranslated Muscle Fibers Skeletal Muscle Proteins Antigens CD34 Cell Count Muscle Development Muscle hypertrophy Mice 0302 clinical medicine Antigens Ly Paired Box Transcription Factors Myocyte Mice Knockout 0303 health sciences education.field_of_study Myogenesis Stem Cells Microfilament Proteins PAX7 Transcription Factor Cell Differentiation musculoskeletal system Cell biology medicine.anatomical_structure Stem cell tissues Satellite Cells Skeletal Muscle Myocytes Smooth Muscle Population Kruppel-Like Transcription Factors Mice Nude Mice Transgenic Biology 03 medical and health sciences medicine Animals Regeneration Cell Lineage Progenitor cell Muscle Skeletal education PAX3 Transcription Factor Cell Proliferation MyoD Protein 030304 developmental biology Myosin Heavy Chains Cell growth Membrane Proteins Proteins Skeletal muscle Cell Biology Actins Mice Inbred C57BL Ki-67 Antigen Animals Newborn 030217 neurology & neurosurgery Stem Cell Transplantation |
| Zdroj: | Nature Cell Biology; Vol |
| ISSN: | 1476-4679 1465-7392 |
| DOI: | 10.1038/ncb2025 |
| Popis: | Satellite cells are resident myogenic progenitors in postnatal skeletal muscle involved in muscle postnatal growth and adult regenerative capacity. Here, we identify and describe a population of muscle-resident stem cells, which are located in the interstitium, that express the cell stress mediator PW1 but do not express other markers of muscle stem cells such as Pax7. PW1(+)/Pax7(-) interstitial cells (PICs) are myogenic in vitro and efficiently contribute to skeletal muscle regeneration in vivo as well as generating satellite cells and PICs. Whereas Pax7 mutant satellite cells show robust myogenic potential, Pax7 mutant PICs are unable to participate in myogenesis and accumulate during postnatal growth. Furthermore, we found that PICs are not derived from a satellite cell lineage. Taken together, our findings uncover a new and anatomically identifiable population of muscle progenitors and define a key role for Pax7 in a non-satellite cell population during postnatal muscle growth. |
| Databáze: | OpenAIRE |
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