Viral genome and antiviral drug sensitivity analysis of two patients from a family cluster caused by the influenza A(H7N9) virus in Zhejiang, China, 2013
| Autor: | Xiao Xin Wu, Nan Ping Wu, Hangping Yao, Hai Nv Gao, Jing Guo, Shu Fa Zheng, Kwok-Yung Yuen, Yi Xin Zhu, Zhang Qiong, Hai Bo Wu, Kun Kai Su, Wei Feng Liang, Honglin Chen, Lanjuan Li, Shi Gui Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Microbiology (medical) China Oseltamivir medicine.medical_specialty medicine.drug_class viruses Genome Viral Biology Influenza A Virus H7N9 Subtype Antiviral Agents Genome Virus lcsh:Infectious and parasitic diseases H7N9 chemistry.chemical_compound Human-to-human transmission Molecular genetics Drug Resistance Viral Influenza Human medicine Humans lcsh:RC109-216 Gene Phylogeny Genetics Neuraminidase inhibitor General Medicine Middle Aged Virology Influenza Infectious Diseases Amino Acid Substitution chemistry Female Peramivir Antiviral drug medicine.drug Drug sensitivity |
| Zdroj: | International Journal of Infectious Diseases, Vol 29, Iss C, Pp 254-258 (2014) |
| ISSN: | 1878-3511 1201-9712 |
| Popis: | Summary Objectives In the winter of 2013, people were facing the risk of human-to-human transmission of the re-emerging influenza A(H7N9) virus. We report herein information on the clinical features of two patients from the same family infected with this virus, the genomic sequences of the viruses harbored, and antiviral drug sensitivity. Methods Clinical and epidemiological data of two patients from the same family were collected and analyzed. Sequencing was done for the viruses isolated from these two patients and one epidemiologically related chicken, and the sequences of the eight gene segments of the viruses were analyzed phylogenetically. The sensitivity of the viruses to antiviral drug treatment was determined by neuraminidase inhibitor susceptibility test. Results The two patients from one family cluster shared the same symptoms but had different outcomes, and had a strong epidemiological link. Three strains, two from these two patients and one from the chicken, were isolated. Genome sequencing and analyses of phylogenetic trees demonstrated that the two viruses were almost identical. We noted the presence of the PB2 E627K amino acid substitution that was not present in isolates from the first wave, as well as two new mutations in the NA gene and six in the PB2 gene. Drug sensitivity testing showed that the new isolates were resistant to oseltamivir but sensitive to peramivir. Conclusions The two patients from one family cluster were probable human-to-human transmission cases. The new isolates were sensitive to peramivir but showed reduced sensitivity to oseltamivir. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|