Hemodynamic changes in hepatic sinusoids of hepatic steatosis mice
Autor: | Jing Fan, Chong-Jiu Chen, Wei Quan, Weiguang Zhang, Yu-Chen Wang, Jian-Wei Wang |
---|---|
Rok vydání: | 2018 |
Předmět: |
Male
medicine.medical_specialty Hepatic steatosis H&E stain Hemodynamics Hepatic sinusoids 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Imaging Three-Dimensional Internal medicine medicine Laser-Doppler Flowmetry Oil Red O Animals Humans Two-photon fluorescence microscopy Carbon tetrachloride Microscopy Confocal Chemistry Microcirculation Fatty liver Gastroenterology General Medicine Blood flow Basic Study medicine.disease Capillaries Fatty Liver Mice Inbred C57BL Red blood cell Disease Models Animal medicine.anatomical_structure Endocrinology Liver 030220 oncology & carcinogenesis 030211 gastroenterology & hepatology Hemorheology Steatosis Blood Flow Velocity |
Zdroj: | World Journal of Gastroenterology |
ISSN: | 2219-2840 |
Popis: | Background Fatty liver (FL) is now a worldwide disease. For decades, researchers have been kept trying to elucidate the mechanism of FL at the molecular level, but rarely involve the study of morphology and medical physics. Traditionally, it was believed that hemodynamic changes occur only when fibrosis occurs, but it has been proved that these changes already show in steatosis stage, which may help to reveal the pathogenesis and its progress. Because the pseudolobules are not formed during the steatosis stage, this phenomenon may be caused by the compression of the liver microcirculation and changes in the hemodynamics. Aim To understand the pathogenesis of hepatic steatosis and to study the hemodynamic changes associated with hepatic steatosis. Methods Eight-week-old male C57BL/6 mice were divided into three groups randomly (control group, 2-wk group, and 4-wk group), with 16 mice per group. A hepatic steatosis model was established by subcutaneous injection of carbon tetrachloride in mice. After establishing the model, liver tissue from mice was stained with hematoxylin and eosin (HE), and oil red O stains. Blood was collected from the angular vein, and hemorheological parameters were estimated. A two-photon fluorescence microscope was used to examine the flow properties of red blood cells in the hepatic sinusoids. Results Oil red O staining indicated lipid accumulation in the liver after CCl4 treatment. HE staining indicated narrowing of the hepatic sinusoidal vessels. No significant difference was observed between the 2-wk and 4-wk groups of mice on morphological examination. Hemorheological tests included whole blood viscosity (mPas, γ = 10 s-1/γ = 100 s-1) (8.83 ± 2.22/4.69 ± 1.16, 7.73 ± 2.46/4.22 ± 1.32, and 8.06 ± 2.88/4.22 ± 1.50), red blood cell volume (%) (51.00 ± 4.00, 42.00 ± 5.00, and 40.00 ± 3.00), the content of plasma fibrinase (g/L) (3.80 ± 0.50, 2.90 ± 0.80, and 2.30 ± 0.70), erythrocyte deformation index (%) (44.49 ± 5.81, 48.00 ± 15.29, and 44.36 ± 15.01), erythrocyte electrophoresis rate (mm/s per V/m) (0.55 ± 0.11, 0.50 ± 0.11, and 0.60 ± 0.20), revealing pathological changes in plasma components and red blood cells of hepatic steatosis. Assessment of blood flow velocity in the hepatic sinusoids with a laser Doppler flowmeter (mL/min per 100 g) (94.43 ± 14.64, 80.00 ± 12.12, and 67.26 ± 5.92) and two-photon laser scanning microscope (μm/s) (325.68 ± 112.66, 213.53 ± 65.33, and 173.26 ± 44.02) revealed that as the modeling time increased, the blood flow velocity in the hepatic sinusoids decreased gradually, and the diameter of the hepatic sinusoids became smaller (μm) (10.28 ± 1.40, 6.84 ± 0.93, and 5.82 ± 0.79). Conclusion The inner diameter of the hepatic sinusoids decreases along with the decrease in the blood flow velocity within the sinusoids and the changes in the systemic hemorheology. |
Databáze: | OpenAIRE |
Externí odkaz: |