Lysophosphatic acid modulates prostaglandin secretion in the bovine uterus
| Autor: | Izabela Woclawek-Potocka, Kiyoshi Okuda, Dariusz J. Skarzynski, Junichi Komiyama, Edyta Brzezicka, Jean Sebastian Saulnier-Blache, Mamadou M. Bah |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Embryology
medicine.medical_specialty Prostaglandin Estrous Cycle Endometrium Dinoprostone Prostaglandin secretion chemistry.chemical_compound Endocrinology Pregnancy Internal medicine Lysophosphatidic acid medicine Animals RNA Messenger Receptors Lysophosphatidic Acid Autocrine signalling Progesterone Prostaglandin-E Synthases LPAR3 LPAR1 Reverse Transcriptase Polymerase Chain Reaction Obstetrics and Gynecology Isoxazoles Cell Biology Intramolecular Oxidoreductases medicine.anatomical_structure Reproductive Medicine chemistry Corpus Luteum Maintenance Hydroxyprostaglandin Dehydrogenases Cattle Female lipids (amino acids peptides and proteins) Lysophospholipids Propionates Corpus luteum |
| Zdroj: | REPRODUCTION. 137:95-105 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-08-0209 |
| Popis: | Lysophosphatidic acid (LPA) modulates prostaglandin (PG) synthesis via LPA receptor 3 (LPAR3) in the murine endometrium. The lack of functional LPAR3 in mice may lead to embryo mortality. In the present study, we examined the role of LPA in the bovine uterus. We confirmed that LPA is locally produced and released from the bovine endometrium. Moreover, there are enzymes involved in LPA synthesis (phospholipase(PL)D2and PLA2G1B) in the bovine endometrium during estrous cycle and early pregnancy. Expression of the receptor for LPA (LPAR1) was positively correlated with the expression ofPGE2synthase(PGES) and negatively correlated with the expression ofPGF2αsynthase(aldose reductase with 20 α-hydroxysteroid dehydrogenase activity –PGFS) during early pregnancy.In vivoLPA induced P4 and PGE2secretion was inhibited by LPAR1 antagonist (Ki16425). The overall results indicate that LPA is locally produced and released from the bovine endometrium. Moreover,LPAR1gene expression in the endometrium during the estrous cycle and early pregnancy indicates that LPA may play autocrine and/or paracrine roles in the bovine uterus.LPAR1gene expression is positively correlated with the expression of the enzyme responsible for luteotropic PGE2production (PGES) in endometrium. In cow, LPA stimulates P4 and PGE2secretion. Thus, LPA in the bovine reproductive tract may indirectly (via endometrium) or directly support corpus luteum action via the increase of P4 synthesis and the increase of PGE2/PGF2αratio. It suggests that LPA may serve as an important factor in the maintenance of early pregnancy in cow. |
| Databáze: | OpenAIRE |
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