Effects of Bushen-Tiaojing-Fang on the pregnancy outcomes of infertile patients with repeated controlled ovarian stimulation
| Autor: | Feng-Li Guan, Yucong Ma, Huilan Du, Na Cui, Guimin Hao, Jing-Wei Chen, Yanli Fan, Yucong Cao, Zhi-Ming Zhao, Jingran Geng, Shuancheng Zhang, Bu-Lang Gao |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Infertility
Adult Molecular biology NF-E2-Related Factor 2 Science Stimulation Diseases medicine.disease_cause Biochemistry Article Andrology Medical research Adenosine Triphosphate Ovulation Induction Pregnancy medicine Humans Embryo Implantation chemistry.chemical_classification Glutathione Peroxidase Multidisciplinary business.industry Superoxide Dismutase Glutathione peroxidase Health care Pregnancy Outcome Embryo medicine.disease Follicular fluid Follicular Fluid Mitochondria chemistry Medicine Dismutase Female business Infertility Female Oxidative stress Drugs Chinese Herbal |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Bushen-Tiaojing-Fang (BSTJF) is commonly used to treat infertility. This study investigated the effects of BSTJF on the pregnancy outcomes of patients with repeated controlled ovarian stimulation (COS), on mitochondrial function, and on oxidative stress in ovarian granulosa cells (GCs) and follicular fluid (FF). The samples and clinical data of 97 patients, including 35 in the control group, 29 in the placebo group and 33 in the BSTJF group, were collected for this study. The mitochondrial ultrastructure, ATP content, mitochondrial DNA (mtDNA) number, 8-hydroxy-2-deoxyguanosine (8-OHdG), Mn-superoxide dismutase (Mn-SOD), glutathione peroxidase (GSH-Px) activity levels, and mRNA expression levels of Mn-SOD, GSH-Px, and nuclear factor erythroid-derived factor 2-related factor 2 (Nrf2) were analyzed. The high-grade embryo (P P = 0.033), and clinical pregnancy (P = 0.031) rates, as well as the ATP content (P = 0.014), mtDNA number (P = 0.035), GSH-Px activity (P = 0.004 in GCs and P = 0.008 in FF) and mRNA expression levels (P = 0.019), were significantly lower in the placebo group than in the control group, whereas the 8-OHdG content was significantly (P = 0.006 in FF) higher in the placebo group than in the control group. Compared with those in the placebo group, the high-grade embryo rate (P = 0.007), antioxidant enzyme activity (P = 0.037 and 0.036 in Mn-SOD; P = 0.047 and 0.030 in GSH-Px) and mRNA level (P P = 0.039 in Mn-SOD and P = 0.002 in GSH-Px) were significantly higher in the BSTJF group, as were changes in mitochondrial ultrastructure, ATP (P = 0.040) and mtDNA number (P = 0.013). In conclusion, BSTJF can improve oxidative stress in patients with repeated COS and pregnancy outcomes. |
| Databáze: | OpenAIRE |
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