Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)
| Autor: | ROSIO G. SCHNEIDER, JUAN M. FERRO, IVANA N. REINKO, JUAN M. BOERIS, DARÍO E. CARDOZO, DIEGO BALDO |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
0106 biological sciences 0301 basic medicine medicine.medical_specialty Science Argentina Zoology C-banding 010603 evolutionary biology 01 natural sciences Chromosomes cytogenetics Giemsa stain XY 03 medical and health sciences medicine Animals In Situ Hybridization Fluorescence heteromorphism Sex Chromosomes Multidisciplinary medicine.diagnostic_test biology Differential staining Leptodactylus bufonius Leptodactylidae heterochromatin Cytogenetics Karyotype biology.organism_classification Leptodactylus Chromosome Banding 030104 developmental biology Female Anura Fluorescence in situ hybridization |
| Zdroj: | Anais da Academia Brasileira de Ciências v.93 n.2 2021 Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências (ABC) instacron:ABC Anais da Academia Brasileira de Ciências, Vol 93, Iss 2 (2021) Anais da Academia Brasileira de Ciências, Volume: 93, Issue: 2, Article number: e20190426, Published: 04 JUN 2021 |
| ISSN: | 1678-2690 0001-3765 |
| DOI: | 10.1590/0001-3765202120190426 |
| Popis: | Cytogenetic analyses were performed on specimens of Leptodactylus bufonius from different localities in Argentina. Mitotic chromosomes were studied with Giemsa and differential staining techniques (Ag-NOR, C-banding, and CMA3/DAPI) and fluorescence in situ hybridization with the 18S DNAr probe. All specimens showed karyotypes with 2n = 2x = 22 and FN = 44. Secondary constrictions were present in the long arm of chromosome pair 8, coincident with Ag-NOR and hybridization signals of the 18S DNAr probe. The C-banding technique evidenced an important amount of heterochromatin with a sex-linked pericentromeric band in the short arm of chromosome pair 4. This heterochromatic band was heteromorphic in males but present in both homologues of females, and it was CMA3 positive (DAPI negative) at fluorescence staining. The occurrence of heteromorphic XY sex chromosomes in L. bufonius is the second known case in Leptodactylus and the fifth within the speciose family Leptodactylidae. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|