Radioactive assay for aryl hydrocarbon hydroxylase. Improved method and biological importance
| Autor: | Jean De Graeve, Jacques Van Cantfort, Jacques Gielen |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Male
Biophysics Kidney Tritium Biochemistry High-performance liquid chromatography chemistry.chemical_compound Animals Molecular Biology Incubation Lung Chromatography High Pressure Liquid chemistry.chemical_classification Aqueous solution Chromatography Cell Biology Aryl Hydrocarbon Hydroxylases Rats Hexane Intestines Enzyme Spectrometry Fluorescence chemistry Liver Isotope Labeling Pyrene |
| Zdroj: | Biochemical and biophysical research communications. 79(2) |
| ISSN: | 0006-291X |
| Popis: | By addition of two volumes of a 1M aqueous KOH/dimethylsulfoxide (1585; v/v) mixture to the enzymatic incubation medium, it is possible to selectively extract the unmetabolized benzo(a)pyrene in hexane. Therefore, the radio-activity remaining in the water phase corresponds to all the in vitro synthesized metabolites. This isotopic method is very sensitive (2 × 10−11 moles) and is almost insensitive to the room lighting. The aryl hydrocarbon hydroxylase activities found with this method are 2,3 and 10 times higher in the liver, lung and kidney respectively compared to those obtained with the fluorimetric method. |
| Databáze: | OpenAIRE |
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