Protection of farm goats by combinations of recombinant peptides and formalin inactivated spores from a lethal Bacillus anthracis challenge under field conditions
| Autor: | Salih Otlu, Wolfgang Beyer, Fatih Büyük, Okechukwu C. Ndumnego, Mitat Şahin, Susanne M. Koehler, Mehmet Doganay, Jens Moehring, Henriette van Heerden, Özgür Çelebi, Hayati Demiraslan |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Turkey Vaccination schedule Bacterial Toxins 030106 microbiology Anthrax Vaccines Biology Microbiology DNA vaccination Anthrax 03 medical and health sciences Immune system Antigen Formaldehyde Animals Spores Bacterial Antigens Bacterial Goat Diseases Membrane Glycoproteins lcsh:Veterinary medicine General Veterinary Goats Immunogenicity fungi Vaccination General Medicine biology.organism_classification Virology Recombinant Proteins Bacillus anthracis Animal vaccine 030104 developmental biology Immunization lcsh:SF600-1100 Peptides Research Article |
| Zdroj: | BMC Veterinary Research, Vol 13, Iss 1, Pp 1-11 (2017) BMC Veterinary Research |
| ISSN: | 1746-6148 |
| DOI: | 10.1186/s12917-017-1140-2 |
| Popis: | Background Bacillus (B.) anthracis, the causal agent of anthrax, is effectively controlled by the Sterne live spore vaccine (34F2) in animals. However, live spore vaccines are not suitable for simultaneous vaccination and antibiotic treatment of animals being at risk of infection in an outbreak situation. Non-living vaccines could close this gap. Results In this study a combination of recombinant protective antigen and recombinant Bacillus collagen-like antigen (rBclA) with or without formalin inactivated spores (FIS), targeted at raising an immune response against both the toxins and the spore of B. anthracis, was tested for immunogenicity and protectiveness in goats. Two groups of goats received from local farmers of the Kars region of Turkey were immunized thrice in three weeks intervals and challenged together with non-vaccinated controls with virulent B. anthracis, four weeks after last immunization. In spite of low or none measurable toxin neutralizing antibodies and a surprisingly low immune response to the rBclA, 80% of the goats receiving the complete vaccine were protected against a lethal challenge. Moreover, the course of antibody responses indicates that a two-step vaccination schedule could be sufficient for protection. Conclusion The combination of recombinant protein antigens and FIS induces a protective immune response in goats. The non-living nature of this vaccine would allow for a concomitant antibiotic treatment and vaccination procedure. Further studies should clarify how this vaccine candidate performs in a post infection scenario controlled by antibiotics. Electronic supplementary material The online version of this article (doi:10.1186/s12917-017-1140-2) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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