Hepatoprotective effects of sericin on aging-induced liver damage in mice
| Autor: | Saeed Sadigh-Eteghad, Kiarash Mokhtari, Alireza Barati, Yasin Bagheri, Javad Mahmoudi, Abdollah Abdollahpour, Zahra Malekinejad, Soheila Montazersaheb, Ezzatollah Fathi, Nasim Jalili Namini |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Aging medicine.medical_treatment medicine.disease_cause Sericin Antioxidants Mice Western blot Oral administration Liver tissue Internal medicine Medicine Animals Liver damage Phosphorylation Sericins Saline Pharmacology medicine.diagnostic_test business.industry Liver Diseases Age Factors JNK Mitogen-Activated Protein Kinases General Medicine Oxidative Stress Endocrinology Gene Expression Regulation Liver Apoptosis business Oxidative stress |
| Zdroj: | Naunyn-Schmiedeberg's archives of pharmacology. 394(12) |
| ISSN: | 1432-1912 |
| Popis: | Aging is a physiological process in which there is a progressive decline of function in multiple organs such as the liver. The development of natural therapies, such as sericin, for delaying age-associated diseases is of major interest in this regard. Twenty-seven mice were divided into three groups of nine, including young control group (8 weeks, received normal saline), aged control group (24 months, received normal saline), and sericin-treated aged mice (24 months, received sericin at dose 100 mg/kg/day) via oral administration for 14 days. The liver enzymes in serum and oxidative stress markers in liver tissue were evaluated using spectrophotometric/ELISA methods. Apoptotic proteins, pro-inflammatory cytokines, COX2, JNK, and P-38 levels were assessed by western blot analysis. β-galactosidase expression was determined by a qRT-PCR method. The findings showed that 100 mg/kg of sericin reduced liver enzymes in aged mice. Antioxidant capacity in treated aged mice showed an improvement in all indexes in the liver tissue. Also, sericin administration declined pro-inflammatory markers to varying degrees in aged-treated mice. Sericin also increased the expression level of Bcl-2 and decreased the expression level of Bax and cleaved caspase-3.In addition, treatment with sericin suppressed protein expression of p-JNK and p-JNK/JNK. Collectively, these findings would infer that sericin administration may have a hepatoprotective effect in aging-induced liver damage in mice. |
| Databáze: | OpenAIRE |
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