A Plausible Proposition of CCL20-Related Mechanism in Fusobacterium nucleatum-Associated Oral Carcinogenesis
Autor: | Swagatika Panda, Abdulaziz A. Al-Kheraif, Divya Gopinath, Sasmita Panda, Darshan Devang Divakar, Neeta Mohanty, Devi Prasad Mandal, Paresh Kumar Behera, Sukumaran Anil |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Science
Population Cell medicine.disease_cause General Biochemistry Genetics and Molecular Biology Article stomatognathic system medicine education Ecology Evolution Behavior and Systematics education.field_of_study biology Fusobacterium nucleatum business.industry Paleontology Cancer medicine.disease biology.organism_classification CCL20 oral squamous cell carcinoma stomatognathic diseases medicine.anatomical_structure Fusobacterium Space and Planetary Science Cancer research Immunohistochemistry business Carcinogenesis |
Zdroj: | Life Volume 11 Issue 11 Life, Vol 11, Iss 1218, p 1218 (2021) |
ISSN: | 2075-1729 |
Popis: | Objective: The objective of this prospective observational case–control study is to evaluate the prevalence of Fusobacterium nucleatum in the tissues of oral squamous cell carcinoma (OSCC). Reconnoitering the CCL20-related mechanism of carcinogenesis in Fusobacterium nucleatum-positive OSCC is another objective. Methodology: Tissues from 50 OSCC patients and 30 healthy oral tissues were collected. The prevalence of Fusobacterium nucleatum was evaluated in both tumour and healthy tissue by polymerase chain reaction. The immunohistochemistry of OSCC tissues was conducted to evaluate the difference in the expression of CCL20 between Fusobacterium nucleatum-positive and -negative OSCC tissues. Results: Fusobacterium nucleatum was significantly (p < 0.001) prevalent in OSCC tissues (74%), compared to healthy tissues (26%). No association of Fusobacterium nucleatum or CCL20 immuno-expression with any clinical or histopathological features of OSCC was observed. While the intensity of CCL20 immuno-expression did not differ (p = 0.053), the CCL20-positive cell population was significantly different (p = 0.034) between Fusobacterium nucleatum-positive and -negative OSCC. Conclusion: Fusobacterium nucleatum is possibly prevalent in oral cancer tissues in the Indian population. By using immunohistochemistry, this is the first study to propose that the carcinogenesis in Fusobacterium nucleatum-positive OSCC may be CCL20-related. The findings enrich the knowledge of mechanisms involved in Fusobacterium nucleatum-mediated oral carcinogenesis. |
Databáze: | OpenAIRE |
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