IL28B, IL29 and micro-RNA 548 in subacute sclerosing panencephalitis as a rare disease
| Autor: | Sevim Karakas Celik, Furuzan Kokturk, Mustafa Calik, Gunes Cakmak Genc, Ahmet Dursun, İbrahim Etem Pişkin |
|---|---|
| Přispěvatelé: | Zonguldak Bülent Ecevit Üniversitesi |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Subacute sclerosing panencephalitis Adolescent IL28B IL29 Disease Polymorphism Single Nucleotide Measles virus Pathogenesis 03 medical and health sciences Rare Diseases 0302 clinical medicine Immune system Genetics medicine Humans Genetic Predisposition to Disease Age of Onset Allele Child 3' Untranslated Regions Allele frequency biology Interleukins fungi Gene polymorphism virus diseases General Medicine biology.organism_classification medicine.disease nervous system diseases MicroRNAs 030104 developmental biology Gene Expression Regulation Case-Control Studies Immunology miRNA 548 Female Interferons 030217 neurology & neurosurgery |
| Zdroj: | Gene. 678:73-78 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2018.07.062 |
| Popis: | Subacute sclerosing panencephalitis (SSPE) is a progressive neurodegenerative disease which affects children and young adults, caused by a persistent infection of defective measles virus. IFN-?s (IL-28A, IL-28B and IL-29) are a group of cytokines mediating antiviral responses. It has been shown that IL-29 levels are significantly higher in infected cells with defective measles virus. IL-29 expression is thought to be regulated at post-transcriptional level and miRNA-548 family targets the 3'UTR of the IFNL1 gene. Impaired immune system has an important role as well as viral factors in SSPE. The aim of our study investigates whether IL-28B, IL-29 levels and gene polymorphisms contribute to the damaged immune response leading to the development of SSPE. Also possible association of miR-548 family with IL-29 and SSPE is explored. Frequencies of rs12979860, rs8099917, rs30461, serum levels of IL-28B, IL-29 and expression levels of miR-548b, miR-548c, miR-548i are determined at 64 SSPE patients and 68 healthy controls. Serum IL-29 levels are statistically significant higher in SSPE patients. Allele frequencies of rs8099917 are statistically significant higher in SSPE patients and resulted G allele is found to increase 2.183-fold risk of SSPE. The expression levels of miR-548b-5p, miR-548c-5p and miR-548i are found to be statistically significant higher in SSPE patients. Dramatically increased level of IL-29 seen in patient group indicates that the elevated miR-548 expression is compensatory result of the over-activated immune system response. Further studies referred to IL28, IL29 and related miRNA's will be enlightened the pathogenesis of SSPE. © 2018 2014-93106430-01 This work was supported by grants from the Bulent Ecevit University, Turkey (Project number: 2014-93106430-01 ). |
| Databáze: | OpenAIRE |
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