Casein Kinase 1 Alpha Regulates Chromosome Congression and Separation during Mouse Oocyte Meiotic Maturation and Early Embryo Development
| Autor: | Jiang-Peng Shen, Cheng-Jie Zhou, Sha-Na Wu, Jie Zhao, Ran Sun, Lu Wang, Angeleem Lu, Cheng-Guang Liang, Hong-Xia Zhou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Anatomy and Physiology
Mouse Cancer Treatment lcsh:Medicine Gene Expression Fluorescent Antibody Technique Biochemistry Chromosome segregation Polar body Mice Molecular cell biology RNA interference Reproductive Physiology Chromosome Segregation lcsh:Science Multidisciplinary Protein Kinase Signaling Cascade Chromosome Biology Imidazoles Casein Kinase Ialpha Genomics Animal Models Chromatin Cellular Structures Signaling Cascades Cell biology Meiosis medicine.anatomical_structure Oncology Benzamides Medicine Epigenetics Female Casein kinase 1 Cellular Types Cell Division Research Article Signal Transduction Blotting Western Embryonic Development Biology Real-Time Polymerase Chain Reaction Signaling Pathways Pyrvinium Compounds Model Organisms Allosteric Regulation medicine Genetics Animals Metaphase Mitosis Germinal vesicle lcsh:R Reproductive System Proteins Chemotherapy and Drug Treatment Oocyte Molecular biology Cytoskeletal Proteins Germ Cells Subcellular Organelles Fertilization Oocytes lcsh:Q Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 5, p e63173 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Casein kinase I alpha (CK1α) is a member of serine/threonine protein kinase, generally present in all eukaryotes. In mammals, CK1α regulates the transition from interphase to metaphase in mitosis. However, little is known about its role in meiosis. Here we examined Ck1α mRNA and protein expression, as well as its subcellular localization in mouse oocytes from germinal vesicle to the late 1-cell stage. Our results showed that the expression level of CK1α was increased in metaphase. Immunostaining results showed that CK1α colocalized with condensed chromosomes during oocyte meiotic maturation and early embryo development. We used the loss-of-function approach by employing CK1α specific morpholino injection to block the function of CK1α. This functional blocking leads to failure of polar body 1 (PB1) extrusion, chromosome misalignment and MII plate incrassation. We further found that D4476, a specific and efficient CK1 inhibitor, decreased the rate of PB1 extrusion. Moreover, D4476 resulted in giant polar body extrusion, oocyte pro-MI arrest, chromosome congression failure and impairment of embryo developmental potential. In addition, we employed pyrvinium pamoate (PP), an allosteric activator of CK1α, to enhance CK1α activity in oocytes. Supplementation of PP induced oocyte meiotic maturation failure, severe congression abnormalities and misalignment of chromosomes. Taken together, our study for the first time demonstrates that CK1α is required for chromosome alignment and segregation during oocyte meiotic maturation and early embryo development. |
| Databáze: | OpenAIRE |
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