In vivo determination of tumor oxygenation during growth and in response to carbogen breathing using 15C5-loaded alginate capsules as fluorine-19 magnetic resonance imaging oxygen sensors
| Autor: | Ulrich Zimmermann, Loreta M. Rodrigues, John R. Griffiths, Barry Newell, Anette Jork, Ulrike Nöth, Simon P. Robinson |
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| Rok vydání: | 2004 |
| Předmět: |
Niacinamide
Radiation-Sensitizing Agents Cancer Research Alginates Partial Pressure Vasodilator Agents Rats Inbred WF chemistry.chemical_compound Glucuronic Acid Carbogen Crown Ethers Neoplasms medicine Animals Radiology Nuclear Medicine and imaging Prolactinoma Tumor microenvironment Radiation Nicotinamide Tumor hypoxia business.industry Hexuronic Acids Fluorine Carbon Dioxide Tumor Oxygenation Hydralazine medicine.disease Magnetic Resonance Imaging Cell Hypoxia Rats Oxygen chemistry Oncology Carbogen Breathing business Nuclear medicine medicine.drug |
| Zdroj: | International Journal of Radiation OncologyBiologyPhysics. 60:909-919 |
| ISSN: | 0360-3016 |
| DOI: | 10.1016/s0360-3016(04)02008-5 |
| Popis: | Purpose The objective was to present a method for the repeated noninvasive measurement of tumor oxygenation (Po 2 ) over the whole period of tumor growth. Methods and materials A mixture of tumor homogenate (GH3 prolactinoma) and alginate capsules loaded with perfluoro-15-crown-5-ether (15C5) was injected into the flanks of Wistar Furth rats. The temporal behavior of tumor Po 2 was monitored between Day 1 and 26 after injection using fluorine-19 ( 19 F) magnetic resonance imaging (MRI). In addition, the response of tumor Po 2 to modifiers of the tumor microenvironment (carbogen [95% O 2 /5% CO 2 ], nicotinamide, and hydralazine) was investigated. Results An initial increase of tumor Po 2 , probably reflecting neovascularization, followed by a decrease after Week 2, probably indicating tumor hypoxia or necrosis, were observed. The minimum and maximum average Po 2 ± SEM observed were 3.3 ± 2.0 mm Hg on Day 2 and 25.7 ± 3.8 mm Hg on Day 13, respectively. Carbogen increased the tumor Po 2 , whereas nicotinamide caused no significant change and hydralazine induced a significant decrease in tumor oxygenation. Conclusions A preclinical method for the repeated noninvasive determination of tumor Po 2 was presented. It might help to investigate tumor physiology and the mechanisms of modifiers of the tumor microenvironment and their role in different therapeutic approaches. |
| Databáze: | OpenAIRE |
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