Quantitative Determination of Coenyzme Q10 by Liquid Chromatography and Liquid Chromatography/Mass Spectrometry in Dairy Products
| Autor: | Mirko Prosek, Monika Strazisar, Veronika Abram, Maja Fir, Luka Milivojevic, Alenka Golc-Wondra |
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| Rok vydání: | 2005 |
| Předmět: |
Time Factors
Ubiquinone Ultraviolet Rays Coenzymes Analytical chemistry Centrifugation Atmospheric-pressure chemical ionization Mass spectrometry Chemistry Techniques Analytical Mass Spectrometry Analytical Chemistry Dioxanes Matrix (chemical analysis) Liquid chromatography–mass spectrometry Animals Environmental Chemistry Sheep milk Chromatography High Pressure Liquid Acetic Acid Pharmacology Detection limit Chromatography Ethanol Molecular mass Clinical Laboratory Techniques Chemistry Temperature Reproducibility of Results Yogurt Milk Solvents Mass spectrum Dairy Products Soybeans Agronomy and Crop Science Food Analysis Chromatography Liquid Food Science |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/88.4.1020 |
| Popis: | The dietary sources of CoQ10 and the evaluation of CoQ10 in dairy products were characterized. For quantitation of CoQ10 in food samples, 2 liquid chromatography (LC) methods with UV and mass spectrometry (MS) detections were developed. LC with UV detection was performed at 25°C on a Hyperclone ODS 5 μm 150 × 4.6 mm column with mobile phase consisting of methanol-ethanol-2-propanol (70 + 15 + 15, v/v/v). Flow rate was 1.0 mL/min. Retention time of CoQ10 was 10.9 ± 0.1 min. The method was sensitive [limit of detection (LOD) = 0.2 mg/kg], reproducible [relative standard deviation (RSD) = 3.0%), and linear up to 25 mg/kg (R > 0.999). LC/MS analysis was performed on a LUNA C18 3 μm, 150 × 4.6 mm column, using mobile phase consisting of ethanol-dioxane-acetic acid (9 + 1 + 0.01, v/v/v), flow rate was 0.6 mL/min, and the retention time of CoQ10 was 4.1 ± 0.1 min. Identification and quantitation were performed with a Finnigan-LCQ mass detector in positive atmospheric pressure chemical ionization mode. Mass spectra were obtained in selected-ion monitoring mode; molecular mass (M+H)+m/z 863.4 ± 1 was used for quantitative determination. MS detection is more sensitive than UV detection (LOD = 0.1 mg/kg), less reproducible (RSD = 4.0%), and linear in selected range. Analytical recoveries are 75–90% and depend on the ratio between the amount of fat in the matrix and the concentration of CoQ10 in the sample. Some soybean milk products were analyzed together with different cow, goat, and sheep milk products. Concentrations obtained with LC and LC/MS were compared with a few accessible results available from the literature. Concentrations varied from 0 ppm in soybean milk to nearly 2 ppm in fresh milk from local farms. |
| Databáze: | OpenAIRE |
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