Differential expression of ST6GAL1 in the tumor progression of colorectal cancer
| Autor: | Aidong Shan, Yan Zhang, Jiaoyang Lu, Zhijue Xu, Sen Zhang, Jianguo Gu, Xue Sun, Yingjiao Xu, Yuguo Du, Bo Feng, Minhua Zheng, Xialin Yan, Yalu Cui, Wei Yan, Xia Zou, Jishun Lu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Programmed cell death Colorectal cancer Biophysics Biology Bioinformatics medicine.disease_cause Biochemistry Gene Expression Regulation Enzymologic 03 medical and health sciences 0302 clinical medicine Antigens CD medicine Tumor Cells Cultured Humans Neoplasm Invasiveness Differential expression Stage (cooking) Molecular Biology Cell Proliferation Cell Biology medicine.disease digestive system diseases Sialyltransferases Gene Expression Regulation Neoplastic 030104 developmental biology Membrane protein Cell culture Tumor progression 030220 oncology & carcinogenesis Cancer research Sialic Acids Carcinogenesis Colorectal Neoplasms |
| Zdroj: | Biochemical and biophysical research communications. 486(4) |
| ISSN: | 1090-2104 |
| Popis: | Elevated expression of β-galactoside α2,6-sialyltranferase 1 (ST6GAL1) has been observed in colorectal cancer (CRC) and demonstrated to be important for its tumorigenesis. Here, we found that ST6GAL1 expression was significantly higher in non-metastatic tumors (stage I and II) than that in metastatic tumors (stage III and IV) using 62 pair-matched tumor/normal tissues. To elucidate the molecular mechanisms of how ST6GAL1 affected the CRC progression, we performed a global identification of the substrates of ST6GAL1 in the colon adenocarcinoma cell line SW480. A total of 318 membrane proteins were identified differentially affected by ST6GAL1 overexpression using metabolic labeling and proteomic analysis. Subsequent bioinformatic analysis revealed a list of potential substrates that might mediate the different functions of ST6GAL1 in CRC including cell movement, cell death and survival. Taken together, these results indicate a dynamic change in the expression of ST6GAL1 during the CRC progression and provide a list of sialylated proteins potentially relevant to the different functions of ST6GAL1 in CRC. |
| Databáze: | OpenAIRE |
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