Oxidative interactions of synthetic lung epithelial lining fluid with metal-containing particulate matter
| Autor: | Kay M. Crissman, Gary E. Hatch, Joel Norwood, Ralph Slade, Guobin Sun, Judy H. Richards |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Pulmonary and Respiratory Medicine
Antioxidant Physiology medicine.medical_treatment Ascorbic Acid Respiratory Mucosa Oxidative phosphorylation In Vitro Techniques Oxygen Isotopes Coal Ash Antioxidants Metal chemistry.chemical_compound Physiology (medical) medicine Humans Pollutant Lung Dose-Response Relationship Drug Chemistry Cell Biology Glutathione Particulates Ascorbic acid Carbon medicine.anatomical_structure Biochemistry Metals visual_art Environmental chemistry visual_art.visual_art_medium Particulate Matter Bronchoalveolar Lavage Fluid Oxidation-Reduction |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 281:L807-L815 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.2001.281.4.l807 |
| Popis: | Epidemiology studies show association of morbidity and mortality with exposure to ambient air particulate matter (PM). Metals present in PM may catalyze oxidation of important lipids and proteins present in the lining of the respiratory tract. The present study investigated the PM-induced oxidation of human bronchoalveolar lavage (BAL) fluid (BALF) and synthetic lung epithelial lining fluid (sELF) through the measurement of oxygen incorporation and antioxidant depletion assays. Residual oil fly ash (ROFA), an emission source PM that contains ∼10% by weight of soluble transition metals, was added (0–200 μg/ml) to BALF or sELF and exposed to 20%18O2 (24°C, 4 h). Oxygen incorporation was quantified as excess 18O in the dried samples after incubation. BALF and diluted sELF yielded similar results. Oxygen incorporation was increased by ROFA addition and was enhanced by ascorbic acid (AA) and mixtures of AA and glutathione (GSH). AA depletion, but not depletion of GSH or uric acid, occurred in parallel with oxygen incorporation. AA became inhibitory to oxygen incorporation when it was present in high enough concentrations that it was not depleted by ROFA. Physiological and higher concentrations of catalase, superoxide dismutase, and glutathione peroxidase had no effect on oxygen incorporation. Both protein and lipid were found to be targets for oxygen incorporation; however, lipid appeared to be necessary for protein oxygen incorporation to occur. Based on these findings, we predict that ROFA would initiate significant oxidation of lung lining fluids after in vivo exposure and that AA, GSH, and lipid concentrations of these fluids are important determinants of this oxidation. |
| Databáze: | OpenAIRE |
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