OmpA and antigenic diversity of bovine Chlamydophila pecorum strains
| Autor: | Max M. Wittenbrink, N. Schmeer, R. Schneider, Bernhard Kaltenboeck, Ernst Heinen |
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| Přispěvatelé: | University of Zurich, Kaltenboeck, B |
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
3400 General Veterinary Molecular Sequence Data Posthitis Cattle Diseases Chlamydiae Microbiology Genetic analysis Enteritis Phylogenetics Molecular genetics medicine Animals Amino Acid Sequence Chlamydophila Infections Phylogeny 10082 Institute of Food Safety and Hygiene Antigens Bacterial Chlamydophila General Veterinary Phylogenetic tree biology 2404 Microbiology Genetic Variation Gene Expression Regulation Bacterial General Medicine medicine.disease biology.organism_classification Virology 570 Life sciences Cattle Bacterial Outer Membrane Proteins |
| DOI: | 10.5167/uzh-14483 |
| Popis: | Infections with the intracellular bacterium Chlamydophila (C.) pecorum are highly prevalent worldwide in cattle. These infections cause significant diseases such as polyarthritis, pneumonia, enteritis, genital infections and fertility disorders, and occasionally sporadic bovine encephalomyelitis. Subclinical respiratory infections of calves with C. pecorum have been associated with airway obstruction, pulmonary inflammation, and reduced weight gains. This investigation examined four chlamydial strains with biological properties of C. pecorum isolated from feces of clinically normal cattle, from calves with pneumonia, and from bulls with posthitis. The objective was to characterize the evolutionary relationships of these bovine chlamydial isolates to other chlamydiae by genetic analysis of the ompA gene, and by the immunological cross-reactivities in Western immunoblot analysis. PCR typing of the ompA gene identified these isolates as C. pecorum. The OmpA-deduced amino acid dissimilarities between these four strains spanned 10-20%. In phylogenetic analysis, the four isolates clustered with C. pecorum ruminant, porcine, and koala strains of different geographic origins rather than with each other. All four isolates showed different patterns of Western immunoblot reactivity with antiserum against bovine C. pecorum strain LW63, and, interestingly, no cross-reactivity of the OmpA proteins with the anti-LW613 OmpA antibodies. These data underscore the polyphyletic population structure of C. pecorum and suggest that the spectrum of C. pecorum OmpA proteins in a host species can occupy the entire evolutionary bandwidth within C. pecorum. The variant immunoblot reactivities support the notion of considerable genomic plasticity of C. pecorum. |
| Databáze: | OpenAIRE |
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