Elucidation of the regulation of an adult cuticle gene Acp65A by the transcription factor Broad
| Autor: | H.-Y. Cui, Matthieu Lestradet, Lynn M. Riddiford, N. Bruey-Sedano, Jean-Philippe Charles |
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| Rok vydání: | 2009 |
| Předmět: |
Gene isoform
Hot Temperature Mutant Response element Molecular Sequence Data Gene expression Genetics Animals Drosophila Proteins Promoter Regions Genetic Molecular Biology Gene Transcription factor Binding Sites biology Base Sequence Pupa Gene Expression Regulation Developmental biology.organism_classification Molecular biology Drosophila melanogaster Insect Science Insect Proteins Integumentary System Drosophila Protein Protein Binding Transcription Factors |
| Zdroj: | Insect molecular biology. 18(4) |
| ISSN: | 1365-2583 |
| Popis: | Broad (BR), an ecdysone-inducible transcription factor, is a major determinant of the pupal stage. The misexpression of BR-Z1 isoform (BR-Z1) during adult development of Drosophila melanogaster prevents the expression of the adult cuticle protein 65A gene (Acp65A). We found that the proximal 237 bp of the 5' flanking region of Acp65A were sufficient to mediate this suppression. A targeted point mutation of a putative BR-Z1 response element (BRE) within this region showed that it was not involved. Drosophila hormone receptor-like 38 (DHR38) is required for Acp65A expression. We found that BR-Z1 repressed DHR38 expression and that BR's inhibition of Acp65A expression was rescued by exogenous expression of DHR38. Thus, BR-Z1 suppresses Acp65A expression by preventing the normal up-regulation of DHR38 at the time of adult cuticle formation. |
| Databáze: | OpenAIRE |
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