Allogeneic mesenchymal stem cells improve the wound healing process of sheep skin

Autor: Lisa Maccatrozzo, Paola Brun, M. Zuin, G. M. DeBenedictis, Jan H. Spaas, Koen Chiers, Marco Vincenzo Patruno, Anna Perazzi, Silvia Ferro, Francesco Gemignani, Ilaria Iacopetti, Chiara Gomiero, Tiziana Martinello, E. Martines
Přispěvatelé: Martinello, T, Gomiero, C, Perazzi, A, Iacopetti, I, Gemignani, F, De Benedictis, G, Ferro, S, Zuin, M, Martines, E, Brun, P, Maccatrozzo, L, Chiers, K, Spaas, J, Patruno, M
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Pathology
BLOOD
medicine
THERAPY
Cell therapy
Neovascularization
0302 clinical medicine
GROWTH-FACTORS
Skin
Mesenchymal stem cell
lcsh:Veterinary medicine
integumentary system
Regenerative
Granulation tissue
Surgical wound
General Medicine
medicine.anatomical_structure
030220 oncology & carcinogenesis
Regenerative medicine
Veterinary (all)
Female
medicine.symptom
Research Article
medicine.medical_specialty
Wound healing
Mesenchymal Stem Cell Transplantation
Real-Time Polymerase Chain Reaction
RATS
Lesion
03 medical and health sciences
REGENERATION
Animals
Veterinary Sciences
REPAIR
Mesenchymal stem cells
Sheep
General Veterinary
TRANSPLANTATION
business.industry
Biology and Life Sciences
CRYOPRESERVATION
MODEL
Transplantation
030104 developmental biology
NUCLEATED CELLS
lcsh:SF600-1100
business
Zdroj: BMC veterinary research 14 (2018): 1–9. doi:10.1186/s12917-018-1527-8
info:cnr-pdr/source/autori:Martinello T.; Gomiero C.; Perazzi A.; Iacopetti I.; Gemignani F.; DeBenedictis G.M.; Ferro S.; Zuin M.; Martines E.; Brun P.; Maccatrozzo L.; Chiers K.; Spaas J.H.; Patruno M./titolo:Allogeneic mesenchymal stem cells improve the wound healing process of sheep skin/doi:10.1186%2Fs12917-018-1527-8/rivista:BMC veterinary research/anno:2018/pagina_da:1/pagina_a:9/intervallo_pagine:1–9/volume:14
BMC VETERINARY RESEARCH
BMC Veterinary Research, Vol 14, Iss 1, Pp 1-9 (2018)
BMC Veterinary Research
ISSN: 1746-6148
DOI: 10.1186/s12917-018-1527-8
Popis: Background Skin wound healing includes a system of biological processes, collectively restoring the integrity of the skin after injury. Healing by second intention refers to repair of large and deep wounds where the tissue edges cannot be approximated and substantial scarring is often observed. The objective of this study was to evaluate the effects of mesenchymal stem cells (MSCs) in second intention healing using a surgical wound model in sheep. MSCs are known to contribute to the inflammatory, proliferative, and remodeling phases of the skin regeneration process in rodent models, but data are lacking for large animal models. This study used three different approaches (clinical, histopathological, and molecular analysis) to assess the putative action of allogeneic MSCs at 15 and 42 days after lesion creation. Results At 15 days post-lesion, the wounds treated with MSCs showed a higher degree of wound closure, a higher percentage of re-epithelialization, proliferation, neovascularization and increased contraction in comparison to a control group. At 42 days, the wounds treated with MSCs had more mature and denser cutaneous adnexa compared to the control group. The MSCs-treated group showed an absence of inflammation and expression of CD3+ and CD20+. Moreover, the mRNA expression of hair-keratine (hKER) was observed in the MSCs-treated group 15 days after wound creation and had increased significantly by 42 days post-wound creation. Collagen1 gene (Col1α1) expression was also greater in the MSCs-treated group compared to the control group at both days 15 and 42. Conclusion Peripheral blood-derived MSCs may improve the quality of wound healing both for superficial injuries and deep lesions. MSCs did not induce an inflammatory response and accelerated the appearance of granulation tissue, neovascularization, structural proteins, and skin adnexa.
Databáze: OpenAIRE
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