Optimization of alkaline protease production from Bacillus subtilis NS isolated from sea water
| Autor: | NS Nisha, J Divakaran |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Protease
Strain (chemistry) biology Magnesium medicine.medical_treatment Sodium chemistry.chemical_element Bacillus subtilis biology.organism_classification 16S ribosomal RNA Applied Microbiology and Biotechnology Marine bacteriophage Biochemistry chemistry Genetics medicine Seawater Food science Agronomy and Crop Science Molecular Biology Biotechnology |
| Zdroj: | African Journal of Biotechnology; Vol 13, No 16 (2014) |
| ISSN: | 1684-5315 |
| Popis: | The protease producing bacterial strain Bacillus subtilis was isolated from sea water and identified by 16S rRNA sequencing. The strain named as B. subtilis NS. Optimization of the strain revealed that the most suitable nitrogen source to enhance protease production was beef extract. Among various carbon sources tested, maximum production of protease was registered in medium with added glucose. The effect of metals ions indicated that maximum protease production was observed in medium supplemented with magnesium chloride (MgCl). Investigating the effect of sodium chloride (NaCl) concentration on protease production revealed that 7% yielded higher protease production. The most suitable pH and temperature for maximum protease production revealed that pH 9 and a temperature of 40°C gave optimal protease production. Keywords: Alkaline protease, Bacillus subtilis , media optimization, marine bacteria African Journal of Biotechnology , Vol 13(16), 1707-1713 |
| Databáze: | OpenAIRE |
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