A Bioseparation Apparatus with High-Pressure Fluid Injection and Fluid Sampling
| Autor: | Lee E. Sciaba, James A. Laugharn, Gustav H. Dreier, Feng Tao, David J. Green, Charles Y. Cheung, Robert A. Hess |
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| Rok vydání: | 1999 |
| Předmět: |
Chromatography
Chemistry Elution Biophysics RNA Cell Biology Buffers Chromatography Ion Exchange Bacteriophage lambda Biochemistry Antibodies Chromatography Affinity Dissociation (chemistry) Separation process chemistry.chemical_compound Antigen High pressure DNA Viral Hydrostatic Pressure Nucleic acid Animals Molecular Biology DNA |
| Zdroj: | Analytical Biochemistry. 269:223-229 |
| ISSN: | 0003-2697 |
| DOI: | 10.1006/abio.1998.2946 |
| Popis: | A novel apparatus in which fluids may be injected and sampled at high pressure is described. Bioseparation applications of the apparatus were demonstrated in three model systems: (1) λDNA was eluted under pressure from an anion exchange column into a low-salt (0.25 M) buffer, thereby eliminating conventional time-consuming desalting procedures required for downstream analysis of the DNA; (2) RNA was separated under pressure from a RNA/DNA mixture, thereby enabling rapid differential preparation of nucleic acids; and (3) an antibody was purified from a protein mixture by affinity capture at one pressure and dissociation from the antigen binding partner at a second pressure, thereby enabling the immunoreactivities of both antibody and antigen to be preserved during the separation process. |
| Databáze: | OpenAIRE |
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