TGF-β1-containing exosomes derived from bone marrow mesenchymal stem cells promote proliferation, migration and fibrotic activity in rotator cuff tenocytes
| Autor: | Cong Xu, Ai Guo, Zheng-Peng Liu, Jia Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
qPCR Quantitative reverse-transcription polymerase chain reaction Fibrotic activity TGF-β1 Transforming growth factors β1 Cell Proliferation Biomedical Engineering Rotator cuff tear Cell morphology Scx Scleraxis Exosomes Tnc Tenascin C Flow cytometry α-SMA α-smooth muscle actin Biomaterials 03 medical and health sciences CCK8 Cell counting kit-8 0302 clinical medicine SDS-PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis BMSC TGF-β1 medicine Col I Collagen I PVDF Polyvinylidene fluoride lcsh:QH573-671 BMSC Bone mesenchymal stem cells Migration lcsh:R5-920 DMEM Dulbecco's modified Eagle's medium medicine.diagnostic_test Chemistry Cell growth lcsh:Cytology Col III Collagen III TGF-βR I/II Transforming growth factors β1 receptor type I/II Transfection Cell cycle Smad7 Mothers against decapentaplegic homolog 7 Microvesicles Cell biology Blot 030104 developmental biology medicine.anatomical_structure FBS Fetal bovine serum Original Article lcsh:Medicine (General) 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Regenerative Therapy, Vol 15, Iss, Pp 70-76 (2020) Regenerative Therapy |
| ISSN: | 2352-3204 |
| Popis: | Objective This study aimed to investigate effects of TGF-β1-containing exosomes derived from bone marrow mesenchymal stem cells (BMSC) on cell function of rotator cuff tenocytes and its implication to rotator cuff tear. Methods The primary BMSC and rotator cuff tenocytes were extracted and cultured. Identification of BMSC were performed by observing cell morphology and measurement of surface biomarkers by flow cytometry. BMSC-derived exosomes were extracted and identified by using electron microscopy, nanoparticle-tracking analysis (NTA) and western blotting. Cell proliferation and cell cycle were measured by CCK-8 assay and flow cytometry assay, respectively. Transwell assay was used for detection of tenocytes migration. The fibrotic activity of tenocytes was determined via qPCR and western blotting assays. Results BMSC and BMSC-derived exosomes were successfully extracted. Treatment of BMSC-derived exosomes or TGF-β1 promoted cell proliferation, migration and increased cell ratio of (S + G2/M) phases in tenocytes, as well as enhanced the expression levels of fibrotic activity associated proteins. However, inhibition of TGF-β1 by transfection of sh-TGF-β1 or treatment of TGFβR I/II inhibitor partially reversed the impact of BMSC-derived exosomes on tenocytes function. Conclusion Taken together, TGF-β1-containing exosomes derived from BMSC promoted proliferation, migration and fibrotic activity in rotator cuff tenocytes, providing a new direction for treatment of rotator cuff tendon healing. |
| Databáze: | OpenAIRE |
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