A solid-phase enzyme immunoassay for anti-insulin antibody in diabetes mellitus patients
| Autor: | Noriaki Kobayashi, Kanshiro Kitagaki, Kashu Shimabukuro, Tadaatsu Ogita, Akira Ghoda, Terumasa Miyamoto, Eiji Terada, Hirokazu Okudaira |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Guinea Pigs Immunology Biology Antibodies Immunoenzyme Techniques Internal medicine Diabetes mellitus medicine Animals Humans Insulin Immunology and Allergy Bovine serum albumin chemistry.chemical_classification medicine.diagnostic_test Antibody titer Radioimmunoassay medicine.disease Diabetes Mellitus Type 1 Enzyme Endocrinology chemistry Immunoassay biology.protein Antibody |
| Zdroj: | Journal of Immunological Methods. 84:245-250 |
| ISSN: | 0022-1759 |
| Popis: | A solid-phase enzyme immunoassay for the measurement of anti-insulin antibodies in the sera of patients with diabetes mellitus was developed. Porcine insulin conjugated with bovine serum albumin was used for coating microtiter plates. This assay was as sensitive as the conventional radioimmunoassay. Anti-insulin antibody titers measured by the enzyme immunoassay and conventional radioimmunoassay correlated well and the correlation coefficient was 0.920, which was statistically significant (P less than 0.001). The enzyme immunoassay detected anti-insulin antibody in 23 out of 35 sera of patients with insulin-dependent diabetes mellitus. The present enzyme immunoassay does not require radioactive materials, is less expensive and is concluded to be practically useful. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|