Plasmodium falciparum Genetic Diversity in Panamá Based on glurp, msp-1 and msp-2 Genes: Implications for Malaria Elimination in Mesoamerica
| Autor: | Dianik Moreno, Vanessa Vásquez, Ana María Santamaría, Carlos Justo, L Romero, José E. Calzada, Luis Fernando Chaves, Azael Saldaña, Chystrie Rigg |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Panamá genotype 030231 tropical medicine Plasmodium falciparum Mesoamerica Parasite load molecular epidemiology General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences 0302 clinical medicine Genotype parasitic diseases medicine Gametocyte lcsh:Science Ecology Evolution Behavior and Systematics Genetics Genetic diversity Molecular epidemiology biology Paleontology biology.organism_classification medicine.disease 030104 developmental biology Space and Planetary Science lcsh:Q Restriction fragment length polymorphism Malaria |
| Zdroj: | Life; Volume 10; Issue 12; Pages: 319 Life Life, Vol 10, Iss 319, p 319 (2020) |
| ISSN: | 2075-1729 |
| DOI: | 10.3390/life10120319 |
| Popis: | Panamá, together with all the nations in Mesoamerica, has committed to eliminate malaria from the region by 2020. As these countries approach malaria elimination and local transmission decreases, an active molecular surveillance to identify genotypes circulating along the border areas is particularly needed to accurately infer infection origin, drug resistance and disease propagation patterns in the region. This study evaluated the genetic diversity and allele frequencies of msp-1, msp-2 and glurp genes using different molecular analyses (nested PCR, PCR-restriction fragment length polymorphism (RFLP) and sequencing) from 106 autochthonous and imported P. falciparum isolates collected from different endemic areas in Panamá between 2003 and 2019. We also explored if P. falciparum genotypes assessed with these molecular markers were associated with relevant malaria epidemiological parameters using a multiple correspondence analysis. A strong association of certain local haplotypes with their geographic distribution in endemic areas, but also with parasite load and presence of gametocytes, was evidenced. Few multiclonal infections and low genetic diversity among locally transmitted P. falciparum samples were detected, consequent with the low transmission intensity of this parasite in Panamá, a pattern likely to be extended across Mesoamerica. In addition, several imported cases were genetically dissimilar to local infections and representative of more diverse extra-continental lineages. |
| Databáze: | OpenAIRE |
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