Biomimetic cues from poly(lactic-co-glycolic acid)/hydroxyapatite nano-fibrous scaffolds drive osteogenic commitment in human mesenchymal stem cells in the absence of osteogenic factor supplements
| Autor: | Justin J. Cooper-White, Mohammad Soheilmoghaddam, Harish Padmanabhan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Mature Bone
Cellular differentiation Simulated body fluid Biomedical Engineering 02 engineering and technology 010402 general chemistry 01 natural sciences Glycols chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Tissue engineering Biomimetics Osteogenesis medicine Humans General Materials Science Lactic Acid Cells Cultured Cell Proliferation Tissue Engineering Tissue Scaffolds Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Osteoblast 021001 nanoscience & nanotechnology 0104 chemical sciences PLGA Durapatite medicine.anatomical_structure Biophysics Cortical bone Cues 0210 nano-technology |
| Zdroj: | Biomaterials Science. 8:5677-5689 |
| ISSN: | 2047-4849 2047-4830 |
| DOI: | 10.1039/d0bm00946f |
| Popis: | Mimicking the complex hierarchical architecture of the 'osteon', the functional unit of cortical bone, from the bottom-up offers the possibility of generating mature bone tissue in tissue engineered bone substitutes. In this work, a modular 'bottom-up' approach has been developed to assemble bone niche-mimicking nanocomposite scaffolds composed of aligned electrospun nanofibers of poly(lactic-co-glycolic acid) (PLGA) encapsulating aligned rod-shape nano-sized hydroxyapatite (nHA). By encoding axial orientation of the nHA within these aligned nanocomposite fibers, significant improvements in mechanical properties, surface roughness, hydrophilicity and in vitro simulated body fluid (SBF) mineral deposition were achieved. Moreover, these hierarchical scaffolds induced robust formation of bone hydroxyapatite and osteoblastic maturation of human bone marrow-derived mesenchymal stem cells (hBMSCs) in growth media that was absent of any soluble osteogenic differentiation factors. The results of this investigation confirm that these tailored, aligned nanocomposite fibers, in the absence of media-bone inductive factors, offer the requisite biophysical and biochemical cues to hBMSCs to promote and support their differentiation into mature osteoblast cells and form early bone-like tissue in vitro. |
| Databáze: | OpenAIRE |
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