Unveiling NIR Aza-Boron-Dipyrromethene (BODIPY) Dyes as Raman Probes: Surface-Enhanced Raman Scattering (SERS)-Guided Selective Detection and Imaging of Human Cancer Cells

Autor:	Danaboyina Ramaiah, Kaustabh Kumar Maiti, Nagappanpillai Adarsh, Adukkadan N. Ramya
Rok vydání:	2017
Předmět:	Boron Compounds Surface Properties Contrast Media Metal Nanoparticles Nanoprobe Nanotechnology Biosensing Techniques 02 engineering and technology Conjugated system Spectrum Analysis Raman 010402 general chemistry 01 natural sciences Antibodies Catalysis Polyethylene Glycols HeLa Structure-Activity Relationship chemistry.chemical_compound symbols.namesake Cell Line Tumor Biomarkers Tumor Humans Particle Size Molecular Structure biology Optical Imaging Organic Chemistry General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences ErbB Receptors Spectrometry Fluorescence chemistry Cell Tracking Colloidal gold Biophysics symbols HT1080 Gold BODIPY 0210 nano-technology Raman spectroscopy Raman scattering
Zdroj:	Chemistry - A European Journal. 23:14286-14291
ISSN:	0947-6539
Popis:	The development of new Raman reporters has attracted immense attention in diagnostic research based on surface enhanced Raman scattering (SERS) techniques, which is a well established method for ultrasensitive detection through molecular fingerprinting and imaging. Herein, for the first time, we report the unique and efficient Raman active features of the selected aza-BODIPY dyes 1-6. These distinctive attributes could be extended at the molecular level to allow detection through SERS upon adsorption onto nano-roughened gold surface. Among the newly revealed Raman reporters, the amino substituted derivative 4 showed high signal intensity at very low concentrations (ca. 0.4 μm for 4-Au). Interestingly, an efficient nanoprobe has been constructed by using gold nanoparticles as SERS substrate, and 4 as the Raman reporter (4-Au@PEG), which unexpectedly showed efficient recognition of three human cancer cells (lung: A549, cervical: HeLa, Fibrosarcoma: HT-1080) without any specific surface marker. We observed well reflected and resolved Raman mapping and characteristic signature peaks whereas, such recognition was not observed in normal fibroblast (3T3L1) cells. To confirm these findings, a SERS nanoprobe was conjugated with a specific tumour targeting marker, EGFR (Epidermal Growth Factor Receptor), a well known targeted agent for Human Fibrosarcoma (HT1080). This nanoprobe efficiently targeted the surface marker of HT1080 cells, threreby demonstrating its use as an ultrasensitive Raman probe for detection and targeted imaging, leaving normal cells unaffected.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30e11bf6e0676fa5b941da7a39a56a21 https://doi.org/10.1002/chem.201702626 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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