Various phosphodiesterase subtypes mediate the in vivo antilipolytic effect of insulin on adipose tissue and skeletal muscle in man
| Autor: | Staffan Enoksson, Eva Degerman, E Hagstrom-Toft, Peter Arner, Valérie Large |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Blood Glucose Glycerol Male Muscle tissue medicine.medical_specialty Microdialysis Phosphodiesterase Inhibitors Lipolysis Vasodilator Agents Endocrinology Diabetes and Metabolism medicine.medical_treatment Adipose tissue White adipose tissue Biology Theophylline Dialysis Solutions Internal medicine Internal Medicine medicine Humans Hypoglycemic Agents Insulin Muscle Skeletal Rolipram Aged Aged 80 and over Ethanol Phosphoric Diester Hydrolases Skeletal muscle Dipyridamole Middle Aged Sterol Esterase Amrinone Pyrrolidinones Isoenzymes medicine.anatomical_structure Endocrinology Adipose Tissue Regional Blood Flow Female medicine.drug |
| Zdroj: | Diabetologia. 41:560-568 |
| ISSN: | 1432-0428 0012-186X |
| DOI: | 10.1007/s001250050947 |
| Popis: | The antilipolytic effect of insulin on human abdominal subcutaneous adipose tissue and skeletal muscle during local inhibition of cAMP-phosphodiesterases (PDEs) was investigated in vivo, by combining microdialysis with a euglycaemic, hyperinsulinaemic clamp. During hyperinsulinaemia, the glycerol concentration decreased by 40 % in fat and by 33 % in muscle. Addition of the selective PDE3-inhibitor amrinone abolished the insulin-induced decrease in adipose glycerol concentration, but did not influence the glycerol concentration in skeletal muscle. Nor did the PDE4-selective inhibitor rolipram or the PDE5-selective inhibitor dipyridamole influence the insulin-induced decrease in muscle tissue glycerol. However, the non-selective PDE-inhibitor theophylline counteracted the antilipolytic action of insulin at both sites. The specific activity of PDEs was also determined in both tissues. PDE3-activity was 36.8 ± 6.4 pmol × min–1× mg–1 in adipose tissue and 3.9 ± 0.5 pmol × min–1× mg–1 in muscle. PDE4-activity in skeletal muscle was high, i. e., 60.7 ± 10.2 pmol × min–1× mg–1 but 8.5 pmol × min–1× mg–1 or less in adipose tissue. In conclusion, insulin inhibits lipolysis in adipose tissue and skeletal muscle by activation of different PDEs, suggesting a unique metabolic role of muscle lipolysis. [Diabetologia (1998) 41: 560–568] |
| Databáze: | OpenAIRE |
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