An EPR study using cyclic hydroxylamines to assess the level of mitochondrial ROS in superinvasive cancer cells
| Autor: | Tania Capeloa, Pierre Sonveaux, Samantha Scheinok, Bernard Gallez, Paolo E. Porporato |
|---|---|
| Přispěvatelé: | UCL - SSS/LDRI - Louvain Drug Research Institute, UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Gene isoform Mitochondrial ROS Biophysics SOD2 Mitochondrion Hydroxylamines Biochemistry Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Hydroxylamine Organophosphorus Compounds Piperidines Superoxides Cell Line Tumor Neoplasms Humans Neoplasm Invasiveness 030102 biochemistry & molecular biology biology Chemistry Superoxide Superoxide Dismutase Electron Spin Resonance Spectroscopy Cell Biology General Medicine Molecular biology female genital diseases and pregnancy complications Mitochondria 030104 developmental biology Cancer cell biology.protein Female Reactive Oxygen Species |
| Zdroj: | Cell biochemistry and biophysics, Vol. 78, no. 3, p. 249-254 (2020) |
| Popis: | It has been proposed that a mitochondrial switch involving a high mitochondrial superoxide production is associated with cancer metastasis. We here report an EPR analysis of ROS production using cyclic hydroxylamines in superinvasive SiHa-F3 compared with less invasive SiHa wild-type human cervix cancer cells. Using the CMH probe, no significant difference was observed in the overall level of ROS between SiHa and SiHa-F3 cells. However, using mitochondria-targeted cyclic hydroxylamine probe mitoTEMPO-H, we detected a significantly higher mitochondrial ROS content in SiHa-F3 compared with the wild-type SiHa cells. To investigate the nature of mitochondrial ROS, we overexpressed superoxide dismutase 2, a SOD isoform exclusively localized in mitochondria, in SiHa-F3 superinvasive cells. A significantly lower signal was detected in SiHa-F3 cells overexpressing SOD2 compared with SiHa-F3. Despite some limitations discussed in the paper, our EPR results suggest that mitochondrial ROS (at least partly superoxide) are produced to a larger extent in superinvasive cancer cells compared with less invasive wild-type cancer cells. |
| Databáze: | OpenAIRE |
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