| Autor: |
Fraser, AJ, Greenland-Bews, C, Kelly, D, Williams, CT, Bengey, D, Buist, K, Clerkin, K, Finch, LS, Gould, S, Kontogianni, K, Savage, HR, Thompson, CR, Wardale, J, Watkins, RL, Wooding, D, Allen, AJ, Body, R, Braybrook, J, Buckle, P, Clark, E, Dark, P, Davis, K, Gordon, A, Hayward, G, Halstead, A, Harden, C, Inkson, C, Jones, N, Jones, W, Lasserson, D, Lee, J, Lendrem, C, Lewington, A, Logan, M, Micocci, M, Nicholson, B, Perera-Salazar, R, Prestwich, G, Price, A, Reynard, C, Riley, B, Simpson, AJ, Tate, V, Turner, P, Wilcox, M, Zhifang, M, Adams, ER, Atienzar, AC, Edwards, T, Allen, DJ |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Fraser, A J, Greenland-Bews, C, Kelly, D, Williams, C T, CONDOR Steering Group, LSTM Diagnostics Group, Body, R, Adams, E R, Cubas Atienzar, A, Edwards, T, Allen, D J & Dark, P 2023, ' A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations ', Scientific Reports, vol. 13, no. 1, 3887 . https://doi.org/10.1038/s41598-023-30754-1 |
| DOI: |
10.1038/s41598-023-30754-1 |
| Popis: |
The emergence of severe acute respiratory syndrome 2 (SARS-CoV-2) variants of concern (VOCs), with mutations linked to increased transmissibility, vaccine escape and virulence, has necessitated the widespread genomic surveillance of SARS-CoV-2. This has placed a strain on global sequencing capacity, especially in areas lacking the resources for large scale sequencing activities. Here we have developed three separate multiplex high-resolution melting assays to enable the identification of Alpha, Beta, Delta and Omicron VOCs. The assays were evaluated against whole genome sequencing on upper-respiratory swab samples collected during the Alpha, Delta and Omicron [BA.1] waves of the UK pandemic. The sensitivities of the eight individual primer sets were all 100%, and specificity ranged from 94.6 to 100%. The multiplex HRM assays have potential as a tool for high throughput surveillance of SARS-CoV-2 VOCs, particularly in areas with limited genomics facilities. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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