Modeling seizure-related behavioral and endocrine phenotypes in adult zebrafish
| Autor: | Allan V. Kalueff, Siddharth Gaikwad, Katie Chang, John DiLeo, Leah Grossman, Eli Utterback, Jonathan Cachat, Keith Wong, Nadine Wu, Christopher Suciu, Adam Stewart, Kevin Frank, Thomas Gilder |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Hydrocortisone Danio Enzyme-Linked Immunosorbent Assay Statistics Nonparametric chemistry.chemical_compound Epilepsy Seizures Internal medicine Caffeine Convulsion medicine Reaction Time Endocrine system Animals Picrotoxin Molecular Biology Zebrafish biology Behavior Animal General Neuroscience Numerical Analysis Computer-Assisted biology.organism_classification medicine.disease Disease Models Animal Endocrinology Phenotype chemistry Pentylenetetrazole Female Neurology (clinical) medicine.symptom Psychology Glucocorticoid Locomotion Developmental Biology medicine.drug |
| Zdroj: | Brain research. 1348 |
| ISSN: | 1872-6240 |
| Popis: | Larval zebrafish (Danio rerio) have recently been suggested as a high-throughput experimental model of epilepsy-related pathogenetic states. Here we use adult zebrafish to study behavioral symptoms associated with drug-evoked seizures. Experimental epilepsy-like states were evoked in zebrafish by exposure for 20 min to three chemoconvulsant drugs: caffeine (250 mg/L; 1.3 mM), pentylenetetrazole (1.5 g/L; 11.0 mM) and picrotoxin (100 mg/L; 0.17 mM). Fish behavior was analyzed using manual and video-tracking methods (Noldus Ethovision XT7). Compared to their respective controls, all three drug-treated groups showed robust seizure-like responses (hyperactivity bouts, spasms, circular and corkscrew swimming) accompanied by elevated whole-body cortisol levels (assessed by ELISA). In contrast, control fish did not display seizure-like behaviors and had significantly lower cortisol levels. Paralleling behavioral and endocrine phenotypes observed in clinical and rodent studies, our data implicates adult zebrafish as an emerging experimental model for epilepsy research. |
| Databáze: | OpenAIRE |
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