Genome-wide distribution of linker histone H1.0 is independent of MeCP2
| Autor: | Laura A. Lavery, Steven Andrew Baker, Huda Y. Zoghbi, Yingyao Shao, Aya Ito-Ishida, Hari Krishna Yamalanchili, Ji-Yoen Kim, Laura Dean Heckman, Zhandong Liu, Laura Marie Lombardi, Yaling Sun |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chromatin Immunoprecipitation congenital hereditary and neonatal diseases and abnormalities endocrine system diseases Methyl-CpG-Binding Protein 2 Polymerase Chain Reaction Genome Article MECP2 Histones Mice 03 medical and health sciences Prosencephalon Histone H1 In vivo mental disorders Animals Cell Nucleus Chemistry General Neuroscience food and beverages DNA Methylation nervous system diseases Cell biology Mice Inbred C57BL 030104 developmental biology Forebrain Excitatory postsynaptic potential Linker Chromatin immunoprecipitation Neuroscience Protein Binding |
| Zdroj: | Nature neuroscience |
| ISSN: | 1546-1726 1097-6256 |
| DOI: | 10.1038/s41593-018-0155-8 |
| Popis: | Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional binding of MeCP2 and H1.0 are largely independent. |
| Databáze: | OpenAIRE |
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