Alteration of histone H3 lysine 9 dimethylation in peripheral white blood cells of septic patients with trauma and cancer
| Autor: | Zhang-Gang Xue, Linghui Jiang, Duming Zhu, Hai-Lei Mao, Yiqin Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Pathology medicine.medical_specialty Fluorescent Antibody Technique immunofluorescence staining Biochemistry Methylation Sepsis sepsis Histones 03 medical and health sciences Histone H3 0302 clinical medicine Neoplasms Histone methylation Genetics medicine Leukocytes Humans Clinical significance esophageal cancer Molecular Biology Aged Aged 80 and over peripheral white blood cells biology Oncogene Lysine C-reactive protein histone 3 lysine 9 dimethylation Cancer Articles Middle Aged medicine.disease 030104 developmental biology trauma C-Reactive Protein Oncology ROC Curve biology.protein Molecular Medicine Wounds and Injuries Female 030217 neurology & neurosurgery |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | The present study aimed to investigate the clinical significance of histone methylation in sepsis. A total of 43 blood samples from trauma and esophageal cancer patients with or without sepsis were collected. Immunofluorescence staining of isolated peripheral white blood cells (WBCs) was conducted. Co-stained 293T cells served as a reference, to allow the levels of histone methylation in different types of WBCs from patients to be determined. Immunostaining analyses revealed different levels of histone 3 lysine 9 dimethylation (H3K9me2) in neutrophils (Neu), lymphocytes (Lym), and monocytes (Mon) from trauma patients. Compared with trauma patients, the levels of H3K9me2 were elevated in the three types of WBCs from cancer patients. When combined with sepsis, trauma patients demonstrated increased H3K9me2 levels in Neu (P=0.0005) and Mon (P=0.0002), whereas cancer patients had a significant decrease of H3K9me2 levels in the three types of WBCs (Neu, P=0.0003; Lym, P=0.007; Mon, P=0.007). The H3K9me2 alterations in patients with trauma and cancer were different with the occurrence of sepsis. A larger cohort study is warranted to explore the diagnostic significance and prognostic implications of altered histone methylation in septic patients. |
| Databáze: | OpenAIRE |
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