Imaging large-scale cellular activity in spinal cord of freely behaving mice
| Autor: | Alexander Arena, Daniela Cook, Kohei J. Sekiguchi, Axel Nimmerjahn, Alexander Ngo, Elizabeth Hoffman, Pavel Shekhtmeyster, Katharina Merten |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell type Cellular activity Fluorescence-lifetime imaging microscopy Science General Physics and Astronomy Mice Transgenic Sensory system Motor Activity Stimulus (physiology) Biology Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine medicine Animals Neurons Microscopy Confocal Multidisciplinary Nociceptors General Chemistry Anatomy Spinal cord Mice Inbred C57BL Posterior Horn Cells 030104 developmental biology medicine.anatomical_structure Spinal Cord Cellular resolution Astrocytes Nociceptor Calcium Mechanoreceptors Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications, Vol 7, Iss 1, Pp 1-13 (2016) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Sensory information from mechanoreceptors and nociceptors in the skin plays key roles in adaptive and protective motor behaviours. To date, very little is known about how this information is encoded by spinal cord cell types and their activity patterns, particularly under freely behaving conditions. To enable stable measurement of neuronal and glial cell activity in behaving mice, we have developed fluorescence imaging approaches based on two- and miniaturized one-photon microscopy. We show that distinct cutaneous stimuli activate overlapping ensembles of dorsal horn neurons, and that stimulus type and intensity is encoded at the single-cell level. In contrast, astrocytes show large-scale coordinated calcium responses to intense but not weak sensory inputs. Sensory-evoked activity is potently suppressed by anaesthesia. By revealing the cellular and computational logic of spinal cord networks under behaving conditions, our approach holds promise for better understanding of healthy and aberrant spinal cord processes. Imaging cellular activity in mouse spinal cord has been historically difficult. Here the authors develop cellular resolution fluorescence imaging approaches in the spinal cord of behaving mice, and report distinct activity patterns of neurons and astrocytes in response to different sensory inputs. |
| Databáze: | OpenAIRE |
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