Safety and utility of image-guided research biopsies in relapsed high-grade serous ovarian carcinoma—experience of the BriTROC consortium

Autor: Andrew R Clamp, Mercedes Jimenez-Linan, Eleanor C Brockbank, Axel Walther, Marcia Hall, Rosalind Glasspool, Hani Gabra, Darren Ennis, Jon Stobo, Geoff Hall, Susan Freeman, Cheryl Wilson, Richard J. Edmondson, Ana Montes, Christina Fotopoulou, Iain A. McNeish, James Paul, Luisa Moore, Sudha Sundar, Teodora Goranova, Liz-Anne Lewsley, Geoff Macintyre, David Kay, Anna M. Piskorz, Charlie Gourley, Michelle Lockley, James D. Brenton
Přispěvatelé: Macintyre, Geoff [0000-0003-3906-467X], Moore, Luiza [0000-0001-5315-516X], Brenton, James [0000-0002-5738-6683], Apollo - University of Cambridge Repository
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Oncology
Cancer Research
DNA Mutational Analysis
CANCER GENOMICS
tagged-amplicon sequencing
Phosphatidylinositol 3-Kinases
0302 clinical medicine
Ovarian carcinoma
License
Peritoneal Neoplasms
Cervical cancer
Aged
80 and over
Ovarian Neoplasms
Manchester Cancer Research Centre
Liver Neoplasms
WOMEN
PHASE-III TRIAL
DNA
Neoplasm
CHEMOTHERAPY
Middle Aged
OPEN-LABEL
ErbB Receptors
Serous fluid
ovarian cancer
Liver
030220 oncology & carcinogenesis
Lymphatic Metastasis
Female
Peritoneum
Life Sciences & Biomedicine
Omentum
EPITHELIAL OVARIAN
Adult
Image-Guided Biopsy
Proto-Oncogene Proteins B-raf
medicine.medical_specialty
image-guided
Class I Phosphatidylinositol 3-Kinases
BEVACIZUMAB
Pain
Proto-Oncogene Proteins p21(ras)
03 medical and health sciences
Breast cancer
Internal medicine
high grade serous carcinoma
Journal Article
Carcinoma
medicine
BREAST-CANCER
Humans
biopsy
Oncology & Carcinogenesis
Lung cancer
methanol fixation
Aged
Science & Technology
MUTATIONS
business.industry
ResearchInstitutes_Networks_Beacons/mcrc
PTEN Phosphohydrolase
medicine.disease
030104 developmental biology
Feasibility Studies
next-generation sequencing
Lymph Nodes
Neoplasm Grading
Tumor Suppressor Protein p53
business
Translational Therapeutics
1112 Oncology And Carcinogenesis
RESISTANCE
Zdroj: British Journal of Cancer
EurOPDX Consortium, Goranova, T, Ennis, D, Piskorz, A M, Macintyre, G, Lewsley, L A, Stobo, J, Wilson, C, Kay, D, Glasspool, R M, Lockley, M, Brockbank, E, Montes, A, Walther, A, Sundar, S, Edmondson, R, Hall, G D, Clamp, A, Gourley, C, Hall, M, Fotopoulou, C, Gabra, H, Freeman, S, Moore, L, Jimenez-Linan, M, Paul, J, Brenton, J D & McNeish, I A 2017, ' Safety and utility of image-guided research biopsies in relapsed high-grade serous ovarian carcinoma-experience of the BriTROC consortium ', British Journal of Cancer . https://doi.org/10.1038/bjc.2017.86
ISSN: 1532-1827
0007-0920
DOI: 10.1038/bjc.2017.86
Popis: BACKGROUND: Investigating tumour evolution and acquired chemotherapy resistance requires analysis of sequential tumour material. We describe the feasibility of obtaining research biopsies in women with relapsed ovarian high-grade serous carcinoma (HGSC).METHODS: Women with relapsed ovarian HGSC underwent either image-guided biopsy or intra-operative biopsy during secondary debulking, and samples were fixed in methanol-based fixative. Tagged-amplicon sequencing was performed on biopsy DNA.RESULTS: We screened 519 patients in order to enrol 220. Two hundred and two patients underwent successful biopsy, 118 of which were image-guided. There were 22 study-related adverse events (AE) in the image-guided biopsies, all grades 1 and 2; pain was the commonest AE. There were pre-specified significant AE in 3/118 biopsies (2.5%). 87% biopsies were fit-for-purpose for genomic analyses. Median DNA yield was 2.87 μg, and was higher in biopsies utilising 14 G or 16 G needles compared to 18 G. TP53 mutations were identified in 94.4% patients.CONCLUSIONS: Obtaining tumour biopsies for research in relapsed HGSC is safe and feasible. Adverse events are rare. The large majority of biopsies yield sufficient DNA for genomic analyses-we recommend use of larger gauge needles and methanol fixation for such biopsies, as DNA yields are higher but with no increase in AEs.British Journal of Cancer advance online publication, 30 March 2017; doi:10.1038/bjc.2017.86 www.bjcancer.com.
Databáze: OpenAIRE
Externí odkaz: