A novel variant genotype C of hepatitis B virus identified in isolates from Australian Aborigines: complete genome sequence and phylogenetic relatedness
| Autor: | Yoshihide Kimura, Etsuro Orito, Tomoyoshi Ohno, Hideaki Kato, Fuminaka Sugauchi, W. G. E. Cooksley, Seiji Suzuki, Ryuzo Ueda, L. A. Butterworth, Masashi Mizokami |
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| Rok vydání: | 2001 |
| Předmět: |
Hepatitis B virus
Genetics Whole genome sequencing Serotype HBsAg Hepatitis B Surface Antigens Native Hawaiian or Other Pacific Islander Genotype Phylogenetic tree Molecular Sequence Data Australia Nucleic acid sequence Biology medicine.disease_cause Virology medicine Animals Humans Amino Acid Sequence Serotyping Gene Phylogeny |
| Zdroj: | Journal of General Virology. 82:883-892 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | There have been no reports of DNA sequences of hepatitis B virus (HBV) strains from Australian Aborigines, although the hepatitis B surface antigen (HBsAg) was discovered among them. To investigate the characteristics of DNA sequences of HBV strains from Australian Aborigines, the complete nucleotide sequences of HBV strains were determined and subjected to molecular evolutionary analysis. Serum samples positive for HBsAg were collected from five Australian Aborigines. Phylogenetic analysis of the five complete nucleotide sequences compared with DNA sequences of 54 global HBV isolates from international databases revealed that three of the five were classified into genotype D and were most closely related in terms of evolutionary distance to a strain isolated from a healthy blood donor in Papua New Guinea. Two of the five were classified into a novel variant genotype C, which has not been reported previously, and were closely related to a strain isolated from Polynesians, particularly in the X and Core genes. These two strains of variant genotype C differed from known genotype C strains by 5·9–7·4% over the complete nucleotide sequence and 4·0–5·6% in the small-S gene, and had residues Arg122, Thr127 and Lys160, characteristic of serotype ayw3, which have not been reported previously in genotype C. In conclusion, this is the first report of the characteristics of complete nucleotide sequences of HBV from Australian Aborigines. These results contribute to the investigation of the worldwide spread of HBV, the relationship between serotype and genotype and the ancient common origin of Australian Aborigines. |
| Databáze: | OpenAIRE |
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