Complex biological patterns of soluble cytokines and CD163 in childhood necessitating age-specific reference intervals for evidence-based clinical interpretation
| Autor: | Zaman Alli, Mary Kathryn Bohn, Alexandra Hall, Kazunori Murata, Lusia Sepiashvili, Khosrow Adeli, Amir Karin |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Aging Pediatrics medicine.medical_specialty Percentile Evidence-based practice Adolescent Clinical Biochemistry Antigens Differentiation Myelomonocytic Receptors Cell Surface Age and sex Article 03 medical and health sciences 0302 clinical medicine Antigens CD Reference Values Humans Medicine Child 030304 developmental biology Sex Characteristics 0303 health sciences business.industry Age Factors Infant General Medicine Age specific 3. Good health Reference intervals Child Preschool 030220 oncology & carcinogenesis Reference values Cytokines Female Automated immunoassay business CD163 |
| Zdroj: | Clin Biochem |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/j.clinbiochem.2021.09.004 |
| Popis: | Background Cytokine measurements to support clinical laboratory and research investigations have become increasingly common in pediatrics. However, there is a paucity of accurate pediatric reference intervals (RIs) essential to the interpretation of cytokine results. To address this gap, here, we establish age- and sex-specific pediatric reference values for clinically relevant inflammatory markers including CD163, and the cytokines IL-1β, IL-6, IL-10, IL-18, TNF-α, IFN-γ, and CXCL-9. Methods Healthy children and adolescents (n = 311, 1–19 years) were recruited as part of the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) study. Multi-analyte measurements in plasma and analytical performance verification were conducted on the ProteinSimple® Ella™ automated immunoassay platform (Bio-Techne, MN, USA). Age- and sex-specific RIs were calculated based on Clinical and Laboratory Standards Institute guidelines. Additionally, 75th and 95th percentile cut-offs were determined. Results Three types of reference value distributions were observed: (a) consistent levels throughout age and sex: IL-6, and IFN-γ, (b) gradual decline in concentration with age: CD163, TNF-α, CXCL-9, and IL-10, (c) significantly higher concentrations during ages 4–14 years than earlier and later ages: IL-1β and IL-18. Reference values for CXCL-9, IL-10, and TNF-α under 8 years of age differed significantly from older children. CD163, IL-18 and IL-1β required three age partitions. CD163 demonstrated significant sex differences in ages 8–13 years. Conclusion The circulating profile of cytokines in children is complex and can vary by age and sex. This necessitates careful interpretation of test results based on age and/or sex specific RIs facilitating more accurate clinical decision making. |
| Databáze: | OpenAIRE |
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