Nucleoid Size Scaling and Intracellular Organization of Translation across Bacteria
| Autor: | Yingjie Xiang, Christine Jacobs-Wagner, Sander K. Govers, William T. Gray, Bradley R. Parry, Sangjin Kim, Manuel Campos |
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| Přispěvatelé: | Yale University [New Haven], Laboratoire de microbiologie et génétique moléculaires (LMGM), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial Cytoplasm [SDV]Life Sciences [q-bio] Population [SDV.BC]Life Sciences [q-bio]/Cellular Biology [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Biology Ribosome Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Organelle medicine Nucleoid Nuclear membrane education Genome size ComputingMilieux_MISCELLANEOUS Cell Size 030304 developmental biology Organelles 0303 health sciences education.field_of_study Bacteria 030306 microbiology Translation (biology) Bacterial nucleoid [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Cellular Structures Cell biology DNA-Binding Proteins medicine.anatomical_structure [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Prokaryotic Cells Protein Biosynthesis Ribosomes 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Cell Cell, Elsevier, 2019, 177 (6), pp.1632-1648.e20. ⟨10.1016/j.cell.2019.05.017⟩ |
| ISSN: | 0092-8674 1097-4172 |
| DOI: | 10.1016/j.cell.2019.05.017⟩ |
| Popis: | SummaryThe scaling of organelles with cell size is thought to be exclusive to eukaryotes. Here, we demonstrate that similar scaling relationships hold for the nucleoid in bacteria. Despite the absence of a nuclear membrane, nucleoid size strongly correlates with cell size, independent of changes in DNA amount and across various nutrient conditions. This correlation is observed in diverse bacteria, revealing a near-constant ratio between nucleoid and cell size for a given species. As in eukaryotes, the nucleocytoplasmic ratio in bacteria varies greatly among species. This spectrum of nucleocytoplasmic ratios is independent of genome size, and instead appears linked to the average cell size of the population. Bacteria with different nucleocytoplasmic ratios have different biophysical properties of the cytoplasm, impacting the mobility and localization of ribosomes. Together, our findings identify new organizational principles and biophysical features of bacterial cells, implicating the nucleocytoplasmic ratio and cell size as determinants of the intracellular organization of translation. |
| Databáze: | OpenAIRE |
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