Oxygen regulates the band 3–ankyrin bridge in the human erythrocyte membrane
| Autor: | Gayani C. Kodippili, Marko Stefanovic, Estela Puchulu-Campanella, Philip S. Low |
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| Rok vydání: | 2012 |
| Předmět: |
Ankyrins
chemistry.chemical_classification Erythrocytes Erythrocyte Membrane EPB41 macromolecular substances Cell Biology Biology Actin cytoskeleton Biochemistry Article Cell biology Oxygen chemistry Anion Exchange Protein 1 Erythrocyte biology.protein Humans Ankyrin Spectrin Cytoskeleton Molecular Biology Band 3 Integral membrane protein Deoxygenation |
| Zdroj: | Biochemical Journal. 449:143-150 |
| ISSN: | 1470-8728 0264-6021 |
| Popis: | The oxygenation state of erythrocytes is known to impact several cellular processes. As the only known O2-binding protein in red blood cells, haemoglobin has been implicated in the oxygenation-mediated control of cell pathways and properties. Band 3, an integral membrane protein linked to the spectrin/actin cytoskeleton, preferentially binds deoxygenated haemoglobin at its N-terminus, and has been postulated to participate in the mechanism by which oxygenation controls cellular processes. Because the ankyrin-binding site on band 3 is located near the deoxyHb (deoxygenated haemoglobin)-binding site, we hypothesized that deoxyHb might impact the association between band 3 and the underlying erythrocyte cytoskeleton, a link that is primarily established through band 3–ankyrin bridging. In the present paper we show that deoxygenation of human erythrocytes results in displacement of ankyrin from band 3, leading to release of the spectrin/actin cytoskeleton from the membrane. This weakening of membrane–cytoskeletal interactions during brief periods of deoxygenation could prove beneficial to blood flow, but during episodes of prolonged deoxygenation, such as during sickle cell occlusive crises, could promote unwanted membrane vesiculation. |
| Databáze: | OpenAIRE |
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