Opioid system mediated anti-nociceptive effect of agomelatine in mice

Autor: Merve Kasap, Özgür Devrim Can
Přispěvatelé: Anadolu Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, Can, Özgür Devrim
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
medicine.drug_class
Receptors
Opioid
mu
Pain
(+)-Naloxone
Motor Activity
Pharmacology
Acetic Acid-Induced Writhing Test
General Biochemistry
Genetics and Molecular Biology
Tail-Clip Test
Mice
03 medical and health sciences
0302 clinical medicine
Opioid receptor
Naltrindole
Receptors
Opioid
delta
Internal medicine
Acetamides
medicine
Animals
Agomelatine
General Pharmacology
Toxicology and Pharmaceutics
Hot plate test
Injections
Intraventricular
Pain Measurement
Analgesics
Dose-Response Relationship
Drug
Naloxone
Chemistry
Receptors
Opioid
kappa
Antagonist
General Medicine
Naltrexone
030104 developmental biology
Nociception
Endocrinology
Opioid
Rotarod Performance Test
Hot-Plate Test
Opioid Receptor
030217 neurology & neurosurgery
medicine.drug
Zdroj: Life Sciences. 163:55-63
ISSN: 0024-3205
DOI: 10.1016/j.lfs.2016.08.031
Popis: WOS: 000385057400006
PubMed ID: 27590609
Aims: This study was planned to examine the antinociceptive efficacy of agomelatine against acute mechanical, thermal, and chemical nociceptive stimuli, as well as to determine the opioid receptor subtypes mediating these effects. Main methods: Tail-clip, hot-plate, and acetic acid-induced writhing tests were performed to evaluate antinociceptive effect. Besides, possible effect of agomelatine on the motor coordination of animals was assessed with a Rota-rod test. Keyfindings: Agomelatine (40 mg/kg and 60 mg/kg) significantly prolonged the reaction time of mice in both the tail-clip and hot-plate tests, suggesting the antinociceptive activity is related to both spinal and supraspinal mechanisms. This drug also reduced the number of writhing behaviors indicating the presence of a peripherally mediated antinociceptive effect Rota-rod testing displayed no notable effect on the motor activity of the animal supporting the conclusion that the observed antinociceptive effect is specific. The agomelatine-induced antinociceptive activity abrogated following pretreatment with naloxone (a non-selective opioid receptor antagonist, 5.48 mg/kg, i.p.), which suggested the participation of opioid mechanisms to the antinociception. The possible contribution of mu, delta and kappa subtypes of opioid receptors to the anti-nociceptive effect were evaluated using naloxonazine (7 mg/kg, s.c.), naltrindole (0.99 mg/kg, i.p.), and nor-binaltorphimine (1.03 mg/kg, i.p.), respectively. Pretreatments using these antagonists abolished the antinociceptive activity of agomelatine in all of the nociceptive test paradigms used, which pointed out that mu, delta, and kappa opioid receptors participated to the action of agomelatine on pain. Significance: These results demonstrated the therapeutic potential of agomelatine in the treatment of pain disorders
Anadolu University Research Foundation (Eskisehir, Turkey) [1404S127]
This work was financially supported by the Anadolu University Research Foundation (Eskisehir, Turkey), project no. 1404S127.
Databáze: OpenAIRE
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